Intragastric hypertonic saline increases vasopressin and central Fos immunoreactivity in conscious rats
Intragastric hypertonic saline increases vasopressin and central Fos immunoreactivity in conscious rats
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DOI：
10.1152/ajpregu.1997.272.3.r750
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发表时间：
1997-03-01
影响因子：
2.8
通讯作者：
Osborn, JW
中科院分区：
文献类型：
--
作者：
Carlson, SH;Beitz, A;Osborn, JW
虽然实验证据支持精氨酸加压素（AVP）释放的外周血管紧张素Ⅱ受体调制，局部渗透信号需要血管紧张素Ⅱ受体激活尚未确定使用生理钠负荷。此外，AVP外周调控的中枢通路尚未明确。本实验观察了生理盐水灌胃对门静脉血渗透压、血浆AVP（P-AVP）和Fos免疫反应性的影响。在麻醉大鼠中，胃内灌注（2.9 ml）高渗（600 mosM）盐水显着增加门静脉渗透压，而全身血液渗透压保持不变。清醒大鼠灌胃高渗盐水可显著升高P-AVP（3.6 +/- 1.3至5.8 +/- 1.9 pg/ml），而在等渗溶液中，血浆渗透压均未观察到变化（296.2 +/- 1.4至297.6 +/- 1.1 mosM）或高渗（291.7 +/- 1.7至291.4 +/- 1.8 mosM）组。最后，灌胃高渗盐水显著增加孤束核（NTS），最后区（AP），外侧臂旁核（LPBN），视上核（SON），和室旁核（PVN）的Fos免疫反应。这些结果表明，胃内高渗盐水产生门静脉渗透信号，触发外周AVP受体刺激AVP释放，同时激活NTS，AP和LPBN除了SON和PVN。
Although experimental evidence supports peripheral osmoreceptor modulation of arginine vasopressin (AVP) release, a local osmotic signal required for osmoreceptor activation has yet to be identified using physiological sodium loads. Additionally, the central pathway involved in peripheral control of AVP has not been clearly established. Experiments were conducted to examine the effect of intragastric saline on portal venous osmolarity, plasma AVP (P-AVP), and Fos immunoreactivity. In anesthetized rats, intragastric infusion (2.9 ml) of hypertonic (600 mosM) saline significantly increased portal venous osmolarity while systemic blood osmolarity remained constant. In conscious rats, intragastric hypertonic saline significantly elevated P-AVP (3.6 +/- 1.3 to 5.8 +/- 1.9 pg/ml), whereas no changes were observed in plasma osmolarity in either the isotonic (296.2 +/- 1.4 to 297.6 +/- 1.1 mosM) or hypertonic (291.7 +/- 1.7 to 291.4 +/- 1.8 mosM) group. Finally, intragastric hypertonic saline significantly increased Fos immunoreactivity in the nucleus of the solitary tract (NTS), area postrema (AP), lateral parabrachial nucleus (LPBN), supraoptic nucleus (SON), and paraventricular nucleus (PVN). These results indicate that intragastric hypertonic saline produces a portal venous osmotic signal that triggers peripheral osmoreceptors to stimulate AVP release while activating the NTS, AP, and LPBN in addition to the SON and PVN.