Preparation and properties of cinnamon-thyme-ginger composite essential oil nanocapsules
Preparation and properties of cinnamon-thyme-ginger composite essential oil nanocapsules
复制标题
肉桂-百里香-生姜复合精油纳米胶囊的制备及性能
DOI：
10.1016/j.indcrop.2018.05.058
复制
发表时间：
2018-10-15
影响因子：
5.9
通讯作者：
Wang, Xuge
中科院分区：
文献类型：
--
作者：
Hu, Jing;Zhang, Yudi;Wang, Xuge
精油（EO）作为天然抗菌剂之一，具有优异的抗菌、抗真菌和抗氧化特性。然而，当EO暴露于氧气、光和热时，其主要成分很容易被氧化变质。本研究以壳聚糖为壁，通过离子凝胶化反应制备了肉桂-百里香-生姜复合精油纳米胶囊（CEO-NPs）。详细研究了壳聚糖（CS）与三聚磷酸盐（TPP）的质量比、复合精油（CEO）以及表面活性剂浓度和pH值对CEO-NPs性能的影响。通过动态光散射（DIS）、傅里叶变换红外光谱（FTIR）、热重分析（TGA）、气相色谱质谱（GC MS）和紫外光谱（UV）测量CEO-NP的形貌和结构。同时，采用抑菌圈法测定了CEO-NPs的抗菌性能。当CS/TPP质量比、CEO和脂肪醇聚氧乙烯醚-9（AEO-9）浓度和pH分别为8:1、1.8 g/L、0.7 g/L和5.26时，得到尺寸为215 nm、zeta电位为25.12 my的CEO-NPs。 FTIR 表明 CEO 已被封装到 NP 中。 TGA显示CEO-NPs的热稳定性较CEO有所提高，CEO的负载量为13.4%。 GC MS 显示，90%以上的CEO成分已被封装在NPs中。 UV表明CEO-NPs具有优异的缓释性能。此外，CEO-NPs 对枯草芽孢杆菌和金黄色葡萄球菌表现出持久的抗菌活性。总之，CEO-NP 可用作潜在的长期天然防腐剂。
Essential oils (EO) as one of natural antimicrobials possess excellent antibacterial, antifungal and antioxidant properties. However, their main components are easy to be oxidated and deteriorated when EO was exposed to the oxygen, light and heat. In this study, cinnamon-thyme-ginger composite essential oil nanocapsules (CEO-NPs) were prepared with chitosan as the wall via ionic gelification reaction. The effect of the mass ratio of chitosan (CS) to tripolyphosphate (TPP), composite essential oils (CEO) and surfactant concentration and pH on the properties of the CEO-NPs were investigated in detail. The morphology and structures of CEO-NPs were measured by dynamic light scattering (DIS), fourier transformation infrared spectroscopy (FTIR), thermo-gravimetric analysis (TGA), gas chromatography mass spectrometry (GC MS) and ultraviolet spectrum (UV). Meanwhile, the antibacterial property of CEO-NPs was determined by inhibition zone method. CEO-NPs with the size of 215 nm and zeta potential of 25.12 my were obtained when the mass ratio of CS/TPP, CEO and fatty alcohol polyoxyethylene ether-9 (AEO-9) concentration and pH were 8:1, 1.8 g/L, 0.7 g/L and 5.26. FTIR demonstrated that CEO has been encapsulated into NPs. TGA showed that the thermal stability of CEO-NPs was improved compared with CEO and the loading capacity of CEO was 13.4%. GC MS displayed that more than 90% ingredients of CEO had been encapsulated into NPs. UV indicated that CEO-NPs had the excellent sustained release property. Furthermore, CEO-NPs showed a long-lasting antibacterial activity against Escherichia Bacillus subtilis and Staphylococcus aweus. In all, CEO-NPs can be used as a potential long-term natural preservative.