High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates.
High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates.
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DOI：
10.1016/j.bmc.2006.04.001
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发表时间：
2006-08
影响因子：
3.5
通讯作者：
K. Totani;I. Matsuo;Y. Ihara;Yukishige Ito
中科院分区：
文献类型：
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作者：
K. Totani;I. Matsuo;Y. Ihara;Yukishige Ito
二氢叶酸还原酶 (DHFR) 的各种高甘露糖型聚糖修饰是通过基于配体的方法实现的，使用聚糖-甲氨蝶呤 (MTX) 缀合物作为 DHFR 的紧密结合聚糖承载配体。由此产生的聚糖-MTX缀合物和相应的人工糖蛋白可用作寡糖和糖蛋白探针，以对凝集素、糖基转移酶或糖苷酶等聚糖识别蛋白进行定量分析。此外，通过结合两种不同类型的聚糖修饰策略，首次开发了具有两种不同的高甘露糖型聚糖的人工糖蛋白。
Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan–methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan–MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing protein such as lectins, glycosyltransferases or glycosidases. Moreover, artificial glycoproteins having two different high-mannose-type glycans were developed for the first time by a combination of two different types of glycan modification strategies.