The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
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DOI：
10.1073/pnas.0500926102
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发表时间：
2005-07-19
影响因子：
11.1
通讯作者：
Frank, J
中科院分区：
文献类型：
--
作者：
Gao, HX;Ayub, MJ;Frank, J
我们目前的分析，冷冻电子显微镜和单粒子重建，从克氏锥虫，动质体原生动物病原体，导致南美锥虫病的80 S核糖体的结构。在此基础上，绘制了T. cruzi 80S核糖体具有遗传学上保守的真核rRNA核心结构，大小亚基结构特征明显。值得注意的是，以前未描述的螺旋结构出现在mRNA出口通道附近的小亚基中。我们认为这种rRNA结构可能参与了核糖体在mRNA 5'端的募集，促进和调节了锥虫特有的翻译起始。
We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5' end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes.