Detection of Saffold viruses from children with acute respiratory infections in Yamagata, Japan, between 2008 and 2015
Detection of Saffold viruses from children with acute respiratory infections in Yamagata, Japan, between 2008 and 2015
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DOI：
10.1002/jmv.24928
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发表时间：
2018-01-01
影响因子：
12.7
通讯作者：
Mizuta, Katsumi
中科院分区：
文献类型：
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作者：
Itagaki, Tsutomu;Aoki, Yoko;Mizuta, Katsumi
尽管萨福尔德病毒 (SAFV) 在 2007 年被报道为一种新型人类心脏病毒，但尚未证明 SAFV 与临床疾病之间存在因果关系，并且尚无 SAFV 的纵向流行病学资料。为了建立SAFV与急性呼吸道感染（ARIs）之间的关系并阐明SAFV的纵向流行病学，2008年至2015年间，从日本山形县的ARIs儿童中收集了7258份鼻咽标本。作为常规监测的一部分，将标本接种在包含六种细胞系的微孔板上，并使用逆转录（RT）-PCR方法对SAFV进行分子筛查。在整个研究期间，检测到 95 例（1.3%）SAFV 基因型 2（SAFV2）和 28 例（0.4%）SAFV3，主要发生在 9 月至 11 月期间。 2009年和2013年发生了两次SAFV2暴发，2012年发生了一次SAFV3暴发，这些暴发期间的阳性率分别为12.1%（53/439）、11%（35/319）和4.4%（20/453）。从患有咽炎、疱疹性咽峡炎和扁桃体炎等急性呼吸道感染的儿童中，检测到 63 株 SAFV2 和 28 株 SAFV3 病毒株。这些结果表明，SAFV2和SAFV3可能是儿童急性呼吸道感染的病原体，其感染主要发生在日本的秋季。
Although Saffold virus (SAFV) was reported as a novel human cardiovirus in 2007, no causative association between SAFV and clinical disease has been proven and the longitudinal epidemiology of SAFVs is not available. To establish the relationship between SAFVs and acute respiratory infections (ARIs) and to clarify the longitudinal epidemiology of SAFVs, 7258 nasopharyngeal specimens were collected from children with ARIs in Yamagata, Japan between 2008 and 2015. The specimens were inoculated on a microplate including six cell lines as part of routine surveillance, and molecular screening was performed for SAFVs using a reverse transcription (RT)-PCR method. Throughout the study period, 95 (1.3%) SAFV genotype 2 (SAFV2), and 28 (0.4%) SAFV3 were detected, mainly between September and November. There were two outbreaks of SAFV2 in 2009 and 2013, and one outbreak of SAFV3 in 2012 and the positive rates during these outbreaks were 12.1% (53/439), 11% (35/319), and 4.4% (20/453), respectively. Sixty-three SAFV2 and 28 SAFV3 strains were detected as a single virus from children with ARIs such as pharyngitis, herpangina, and tonsillitis. These results suggested that SAFV2 and SAFV3 are possible causative agents of ARIs among children and their infections occur mainly in the autumn season in Japan.