Peptide products of the afp-6 gene of the nematode Ascaris suum have different biological actions.
Peptide products of the afp-6 gene of the nematode Ascaris suum have different biological actions.
复制标题
猪蛔虫线虫afp-6基因的肽产物具有不同的生物学作用。
DOI：
10.1002/cne.21357
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Stretton,AntonyO
中科院分区：
文献类型：
--
作者：
Yew,JoanneY;Davis,Ralph;Dikler,Sergei;Nanda,Jennifer;Reinders,Barbara;Stretton,AntonyO
使用基质辅助激光解吸/电离飞行时间和串联飞行时间（MALDI-TOF 和 MALDI-TOF/TOF）质谱法对猪蛔虫神经系统中三种新型相关神经肽 AMRNALVRFamide (AF21)、NGAPQPFVRFamide (AF22) 和 SGMRNALVRFamide (AF23) 进行测序和定位。氨基酸序列用于克隆一种新型神经肽基因 (afp-6)，该基因编码带有每个肽的单个副本的前体。原位杂交和免疫细胞化学显示转录物和肽均在腹侧神经节的单个细胞中表达。对注射这些肽的完整线虫的药理学研究，以及对肌肉组织、运动神经元和咽部对它们的反应的生理学研究表明，这些肽在运动和摄食系统中具有有效的生物活性。进一步探索它们的作用可能有助于我们理解神经肽对行为的调节，也有助于开发具有驱虫作用的化合物。 J.Comp。内罗尔。 502:872–882, 2007。© 2007 Wiley‐Liss, Inc.
Matrix‐assisted laser desorption/ionization time‐of‐flight and tandem time‐of‐flight (MALDI‐TOF and MALDI‐TOF/TOF) mass spectrometry were used to sequence and localize three novel, related neuropeptides in the nervous system of the nematodeAscaris suum, AMRNALVRFamide (AF21), NGAPQPFVRFamide (AF22), and SGMRNALVRFamide (AF23). The amino acid sequences were used to clone a novel neuropeptide gene (afp‐6)that encodes a precursor bearing a single copy of each of the peptides. In situ hybridization and immunocytochemistry revealed that both the transcript and the peptides are expressed in a single cell in the ventral ganglion. Pharmacological studies of intact nematodes injected with these peptides, as well as physiological studies of responses to them in muscle tissue, motor neurons, and the pharynx, reveal that these peptides have potent bioactivity in the locomotory and feeding systems. Further exploration of their effects may contribute to our understanding of neuropeptide modulation of behavior and also to the development of compounds with anthelmintic relevance. J. Comp. Neurol. 502:872–882, 2007. © 2007 Wiley‐Liss, Inc.