Optimizing the cationic conjugated polymer-sensitized fluorescent signal of dye labeled oligonucleotide for biosensor applications
Optimizing the cationic conjugated polymer-sensitized fluorescent signal of dye labeled oligonucleotide for biosensor applications
复制标题
DOI：
10.1016/j.bios.2008.07.029
复制
发表时间：
2009-01-01
影响因子：
12.6
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Pu, Kan-Yi;Liu, Bin
用于真实的时间、高选择性和灵敏的多核苷酸检测的方法具有巨大的科学和经济重要性。基于荧光共振能量转移（FRET）的分析利用水溶性共轭聚合物（CP）的集体响应和水性聚电解质的自组装特性，已广泛用于DNA、RNA、蛋白质和小分子的检测。CP基生物传感器的检测灵敏度取决于CP激发时染料发射相对于直接激发时染料发射的信号放大。使用阳离子聚芴衍生物和发色团（荧光素或德克萨斯红）标记的单链DNA分子（ssDNA-C*）作为供体/受体对，我们表明，除了光谱重叠，取向和供体和受体之间的距离，聚合物/DNA-C* 复合物内的供体/受体的能级和荧光猝灭也是影响染料发射信号输出的重要因素。(c)2008 Elsevier B. V.保留所有战斗
Methods for real time, highly selective and sensitive polynucleotide detection are of vast scientific and economic importance. Fluorescence resonance energy transfer (FRET)-based assays which take advantage of the collective response of water-soluble conjugated polymers (CPs) and the self-assembly characteristic of aqueous polyelectrolytes have been widely used for the detection of DNA, RNA, protein and small molecules. The detection sensitivity of CP-based biosensor is dependent on the signal amplification of dye emission upon excitation of CP relative to that upon direct excitation of the dye. Using cationic polyfluorene derivatives and chromophore (fluorescein or Texas Red) labeled single-stranded DNA molecules (ssDNA-C*) as donor/acceptor pairs, we show that in addition to the spectral overlap, orientation and distance between the donor and the acceptor, the energy levels and fluorescence quenching of the donor/acceptor within the polymer/DNA-C* complexes are also important factors that affect the signal output of dye emission. (c) 2008 Elsevier B.V. All Fights reserved