Labeling stem cells with superparamagnetic iron oxide nanoparticles: analysis of the labeling efficacy by microscopy and magnetic resonance imaging.
Labeling stem cells with superparamagnetic iron oxide nanoparticles: analysis of the labeling efficacy by microscopy and magnetic resonance imaging.
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DOI：
10.1007/978-1-61779-953-2_18
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Mendez-Otero, Rosalia
中科院分区：
文献类型：
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作者：
Jasmin;Torres, Ana Luiza Machado;Jelicks, Linda;de Carvalho, Antonio Carlos Campos;Spray, David C;Mendez-Otero, Rosalia
干细胞疗法已成为细胞死亡相关心脏病的潜在治疗选择。非侵入性细胞跟踪方法的应用对于确定干细胞注射后的组织分布和寿命是必要的，并且可能提供关于组织修复的干细胞机制的知之甚少的知识。磁共振成像（MRI）是一种潜在的优秀工具，用于高分辨率可视化移植后细胞的命运和评估治疗策略。MRI用于体内细胞追踪的应用需要造影剂来实现有效的细胞标记，而不会引起任何毒性细胞效应或引起任何其他副作用。由于这些原因，临床上批准的造影剂（例如，氧化铁）和掺入促进剂（例如，鱼精蛋白）是目前用于细胞标记和追踪的优选材料。在这里，我们描述了如何使用超顺磁性氧化铁纳米粒子标记细胞，并在几种疾病模型中监测细胞的命运。
Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamag-netic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.