Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso.
Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso.
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DOI：
10.12688/wellcomeopenres.14932.1
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Goncalves, Bronner P
中科院分区：
文献类型：
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作者：
Barry, Aissata;Behet, Marije C;Goncalves, Bronner P
背景：生活在疟疾流行地区的人对严重疟疾具有自然获得性免疫，但尚不清楚影响感染建立的免疫是否在持续自然暴露后产生。方法：我们清除了布基纳法索学龄儿童可能的亚专利感染，并通过聚合酶链式反应每周检查他们是否有偶发感染。在登记时收集的血浆样本用于定量检测红细胞前期抗原、环子孢子蛋白(CSP)和肝期抗原的抗体。用恶性疟原虫NF54子孢子评价自然获得抗体对子孢子滑动的抑制作用；用人HC-04肝癌细胞株和NF54子孢子评价肝细胞侵袭能力。研究了这些功能性红细胞前免疫表型与聚合酶链式反应检测感染时间之间的关系。结果：共监测51名儿童，首次通过聚合酶链式反应检测到感染或出现临床症状的中位时间为28天。抗CSP抗体滴度与子孢子滑动运动抑制呈显著正相关(P
Background: Individuals living in malaria-endemic regions develop naturally acquired immunity against severe malarial disease, but it is unclear whether immunity that affects the establishment of infections develops following continuous natural exposure. Methods: We cleared schoolchildren in Burkina Faso of possible sub-patent infections and examined them weekly for incident infections by PCR. Plasma samples collected at enrolment were used to quantify antibodies to the pre-eryhrocytic-stage antigens circumsporozoite protein (CSP) and liver stage antigen. Sporozoite gliding inhibition by naturally acquired antibodies was assessed using Plasmodium falciparum NF54 sporozoites; hepatocyte invasion was assessed using the human HC-04 hepatoma cell line and NF54 sporozoites. The associations between these functional pre-erythrocytic immunity phenotypes and time to PCR-detected infection were studied. Results: A total of 51 children were monitored; the median time to first detection of infection by PCR or development of clinical symptoms was 28 days. Anti-CSP antibody titres showed a strong positive association with sporozoite gliding motility inhibition (P