In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses
In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses
复制标题
DOI：
10.1073/pnas.0405316102
复制
发表时间：
2005-01-25
影响因子：
11.1
通讯作者：
Beaurepaire, E
中科院分区：
文献类型：
--
作者：
Supatto, W;Débarre, D;Beaurepaire, E
利用飞秒激光脉冲诱导消融结合多模态非线性显微技术，在体内研究了与胚胎发育相关的复杂生物力学事件。我们展示了受控的活体消融，保留了局部细胞骨架动力学，并导致非突变果蝇胚胎中特定形态发生运动的调节。在表达gfp的系统中，使用全胚胎双光子显微镜和在未标记的非转基因胚胎中，使用三次谐波显微镜获得了复杂运动的定量描述。该方法通过揭示原肠形成时气孔细胞中扭曲蛋白表达与体内组织变形模式的相关性，为机械敏感基因表达问题提供了见解。
The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.