Acute and early life stage toxicity of industrial effluent on Japanese medaka (Oryzias latipes).
Acute and early life stage toxicity of industrial effluent on Japanese medaka (Oryzias latipes).
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DOI：
10.1016/j.scitotenv.2005.04.038
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发表时间：
2006-03
期刊：
影响因子：
--
通讯作者：
Jinmiao Zha;Zijian Wang
中科院分区：
文献类型：
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作者：
Jinmiao Zha;Zijian Wang
为建立全毒性试验方法，对某印钞厂（BPP）废水进行了日本稻鳉鱼（Oryzias latipes）胚胎幼虫期毒性试验。该方法的急性毒性以96 h幼虫道德为终点，慢性毒性以孵化时间、孵化成功率、畸形、生长速度、游泳失败、累计死亡率和性别比为终点。96 h幼虫死亡率试验中，LC50为72.9%（浓度对50%的新孵化medaka幼虫致死）。在慢性毒性试验中，在25±1℃的24 h静态更新系统中，将小于5 h的新受精胚胎暴露于1%、6.25%、12.5%、25%、50%的污水浓度和200 μg/l的BPA中，直至孵化后15天。结果显示，稀释50%时，所有慢性终点均与对照组有显著差异（p<0.01）。胚胎在高浓度（12.5%,25%，50% BPP出水浓度）下第4天开始出现病变。25%稀释处理组孵化时间延迟。在稀释25%的情况下，男性和女性的体重都有所下降。在BPP出水浓度为25%和50%时，新孵化的幼虫出现畸形。稀释25%时，幼虫雌雄比例交替，出现雌性化现象。我们认为，利用medaka进行胚胎幼虫期试验可以评估工业废水的急性和慢性毒性以及潜在的内分泌干扰活性。
To develop the whole effluent toxicity testing methods (WET), embryo larval stage toxicity test using Japanese medaka (Oryzias latipes) was conducted to evaluate an effluent from a banknote printing plant (BPP). The method is based on acute toxicity using endpoint of 96-h larval morality and on chronic toxicity using endpoints such as the time to hatch, hatching success, deformity, growth rate, swim-up failure, accumulative mortality and sexual ratio. In test for 96-h larval mortality, LC50 (the concentration was lethal to 50% of newly hatching medaka larvae) was 72.9%. In chronic toxicity test, newly fertilized embryos (<5-h old) were exposed to 1%, 6.25%, 12.5%, 25%, 50% effluent concentrations and to 200 μg/l BPA in a 24-h static renewal system at 25±1 °C until 15 day post-hatch. The results showed that all chronic endpoints were significantly different from the control at 50% dilution (p<0.01). Embryos began to show lesions on 4th day at higher concentrations (12.5%, 25%, 50% BPP effluent concentrations). Treatment group of 25% dilution showed delayed time to hatch. A reduction in body weight was observed at 25% dilutions for males and females, respectively. Deformities were observed in newly hatched larvae at 25% and 50% BPP effluent concentrations. At 25% dilution, sex ratio of larvae was alternated and there was feminization phenomenon. We conclude that embryo larval stage test using medaka is feasible to evaluate both acute and chronic toxicities and potential endocrine disrupting activity of industrial effluents.