A Novel Skin Splint for Accurately Mapping Dermal Remodeling and Epithelialization During Wound Healing
A Novel Skin Splint for Accurately Mapping Dermal Remodeling and Epithelialization During Wound Healing
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DOI：
10.1002/jcp.25595
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发表时间：
2017-06-01
影响因子：
5.6
通讯作者：
Hara, Shuuji
中科院分区：
文献类型：
--
作者：
Jimi, Shiro;De Francesco, Francesco;Hara, Shuuji
用硅夹板固定小鼠背切创面全层创面模型已被广泛应用于模拟人体创面愈合。然而，由于创面上皮化的起始点尚不清楚，该方法无法准确量化皮肤重塑。为了克服这一限制，我们开发了一种新的小鼠切除伤口模型，使用固定在周围表皮组织下的塑料环形夹板来评估上皮延伸和再生的程度。实验结束后，将组织标本用福尔马林固定，取下夹板，制作石蜡切片。夹板孔在组织横截面上明显，与夹板先前的位置相对应，孔上方的表皮被认为是初始切除部位。表皮收缩和上皮再生作为伤口愈合过程中独立的重要组织改变，是可以区分和量化的。与以往的夹板模型相比，该方法可以准确地评估创面愈合过程中的表皮过程，并可作为评估各种创面愈合因素影响的平台。(C) 2016 Wiley期刊公司
The mouse excisional dorsal full-thickness wound model with a silicon splint fixed on the skin has been widely used to mimic human wound healing. However, the method cannot accurately quantify dermal remodeling, since the initial point of epithelialization on the wound surface is unclear. To overcome this limitation, we have developed a novel mouse excisional wound model to assess the degree of epithelial extension and regeneration, using a plastic ring-shaped splint fixed beneath the surrounding epidermal tissue. At the end of the experiment, tissue samples were fixed in formalin, the splint was excised, and paraffin sections were prepared. Splint holes, corresponding to the prior location of the splint, were evident on the tissue cross-sections, and the epidermis above the holes was considered the initial excision site. The epidermal contraction and epithelial regeneration, as independent essential tissue alterations in wound healing, could be distinguishable and quantified. Compared with previous splint models, this method provides an accurate evaluation of epidermal processes in wound healing, and can be a platform to assess the effects of various wound healing factors. (C) 2016 Wiley Periodicals, Inc.