Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications
Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications
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DOI：
10.1073/pnas.0711421105
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发表时间：
2008-06-10
影响因子：
11.1
通讯作者：
Uenlue, M. Selim
中科院分区：
文献类型：
--
作者：
Oezkumur, Emre;Needham, James W.;Uenlue, M. Selim
直接监测初级分子结合相互作用而不需要二级反应物将显著简化和扩展高通量无标记检测方法的应用。提出了一种简单的干涉测量技术，用于监测生物分子质量累积产生的光学相位差。作为一个例子，动态监测由BSA、人血清白蛋白、兔IgG和蛋白G组成的四种蛋白质中的每一种的50个点，因为它们捕获了相应的抗体。动态测量在26 pg/mm（2）SD/斑点和可检测浓度为19 ng/ml。所提出的方法是特别相关的蛋白质微阵列分析，因为它是无标记，简单，灵敏，容易扩展到高通量。
Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm(2) SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.