MORPHOMETRIC ANALYSIS OF HEPATOCELLULAR-CARCINOMA
MORPHOMETRIC ANALYSIS OF HEPATOCELLULAR-CARCINOMA
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DOI：
10.1007/bf00716991
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发表时间：
1988-01-01
期刊：
影响因子：
--
通讯作者：
KONDO, Y
中科院分区：
文献类型：
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作者：
KONDO, F;WADA, K;KONDO, Y
为探讨高分化肝细胞癌的细胞学特征，对30例高分化肝细胞癌和正常对照组(正常肝、肝硬变、萎缩性肝)进行了比较形态计量学研究。在小梁型肝癌中，正常小梁亚型(1-2个细胞厚的细胞板)通常表现为最小的细胞学异型性，被归类为高分化或高分化型肝癌。应用图像分析仪对肝细胞的平均大小()、平均核大小()、核浆比(N/C)和变异系数(CV)进行量化分析。在正常小梁型肝细胞癌中，与正常和肝硬变相比略有降低，但显著降低(t检验：P<；0.005)。在中、大梁性肝癌中，其值进一步降低。正常肝小梁细胞与正常肝和肝硬变肝细胞的N/C值几乎相同，但N/C值显著高于对照组(t检验：P<；0.001)。在其他类型的碳氢化合物中，N/C比变得更大。在其他肝细胞癌组和对照组中，变异系数相对恒定，而在多形性肝细胞癌和肝细胞异型增生中变异系数极高。结果表明，在组织学标本中，N/C比值的降低和升高实际上发生在分化较好的肝细胞癌中。对于分化良好的肝细胞癌的组织学诊断，考虑到这些观察结果，检查肝标本将是非常重要的。
In order to characterize the cytological features of highly differentiated hepatocellular carcinoma (HCC), a comparative morphometric study was made by observing 30 cases of HCCs and controls (normal, cirrhotic, and atrophic livers). Among trabecular HCCs, normotrabecular subtype (1–2 cell thick cell plate) usually showed minimal cytological atypism and was categorized as well or highly differentiated HCC. Using an image analyzer, the following 4 parameters were applied to quantitate the hepatocyte changes: mean cell size (), mean nuclear size (), nucleocytoplasmic (N/C) ratio and a coefficient of variance (CV = index of anisokaryosis). In normotrabecular HCCs,was slightly but significantly reduced when compared with normal and cirrhotic livers (t-test:p<0.005). The value was further reduced in mid- and macrotrabecular HCCs. Normotrabecular HCCs showed almost the samevalue as normal and cirrhotic livers but displayed significantly a higher N/C value than those of controls (t-test:p<0.001). The N/C ratio became even greater in other types of HCCs. While CV was relatively constant in other HCC groups and controls, it was extremely high in the pleomorphic type of HCC and liver cell dysplasia.The results indicated that a reduction inand increase in N/C ratio, which appear as “nuclear crowding” in histological specimens, actually occurs in well differentiated HCC. For the histologic diagnosis of well differentiated HCC, it would be very important to examine liver specimens with these observations in mind.