The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10
The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10
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DOI：
10.1016/s0378-1097(03)00609-8
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发表时间：
2003-09-12
影响因子：
2.1
通讯作者：
Stolz, JF
中科院分区：
文献类型：
--
作者：
Afkar, E;Lisak, J;Stolz, JF
从革兰氏阳性、嗜卤碱菌、还原硒芽孢杆菌菌株MLS 10中纯化并表征呼吸性砷酸盐还原酶。它是一种膜结合的异源二聚体（150 kDa），由两个亚基ArrA（110 kDa）和ArrB（34 kDa）组成，对砷酸盐的表观Km为34 μ M，V-max为2.5 μ mol min（-1）mg（-1）。最佳活性发生在pH 9.5和150克升（-1）的NaCl。金属分析（电感耦合等离子体质谱法）的全酶和催化亚基（ArrA的基因克隆和测序）的序列分析表明，它是一个成员的DMSO还原酶家族的糖蛋白。(C)2003年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
The respiratory arsenate reductase from the Gram-positive, haloalkaliphile, Bacillus selenitireducens strain MLS10 was purified and characterized. It is a membrane bound heterodimer (150 kDa) composed of two subunits ArrA (110 kDa) and ArrB (34 kDa), with an apparent K-m for arsenate of 34 muM and V-max of 2.5 mumol min(-1) mg(-1). Optimal activity occurred at pH 9.5 and 150 g l(-1) of NaCl. Metal analysis (inductively coupled plasma mass spectrometry) of the holoenzyme and sequence analysis of the catalytic subunit (ArrA; the gene for which was cloned and sequenced) indicate it is a member of the DMSO reductase family of molybdoproteins. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.