The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays.
The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays.
复制标题
DOI：
10.1101/gad.279406.116
复制
发表时间：
2016-07-01
影响因子：
10.5
通讯作者：
Cyster JG
中科院分区：
文献类型：
--
作者：
Han BY;Foo CS;Wu S;Cyster JG
Here, Han et al. show that transcription factor Zfp335 binds DNA and drives transcription via recognition of two distinct consensus motifs by separate ZF clusters and identify the specific motif interaction disrupted by the mutation R1092W. This study presents Zfp335 as a model for understanding how C2H2-ZF TFs may use multiple recognition motifs to control gene expression. The complexities of DNA recognition by transcription factors (TFs) with multiple Cys2–His2 zinc fingers (C2H2-ZFs) remain poorly studied. We previously reported a mutation (R1092W) in the C2H2-ZF TF Zfp335 that led to selective loss of binding at a subset of targets, although the basis for this effect was unclear. We show that Zfp335 binds DNA and drives transcription via recognition of two distinct consensus motifs by separate ZF clusters and identify the specific motif interaction disrupted by R1092W. Our work presents Zfp335 as a model for understanding how C2H2-ZF TFs may use multiple recognition motifs to control gene expression.
