Construction of a semisynthetic antibody library using trinucleotide oligos
Construction of a semisynthetic antibody library using trinucleotide oligos
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DOI：
10.1093/nar/25.22.4690
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发表时间：
1997-11-15
影响因子：
14.9
通讯作者：
Little, M
中科院分区：
文献类型：
--
作者：
Braunagel, M;Little, M
通过使用三核苷酸密码子将人scFv的重链CDR 3区用8个氨基酸的随机序列替换来构建由单链Fv片段（scFv）组成的半合成抗体文库。在克隆到噬菌体展示载体中之后，产生具有8 × 10（8）个独立克隆的复杂性的抗体文库。异硫氰酸荧光素和3-硝基-4-羟基-5-碘苯基-乙酸，在每种情况下获得特异性结合抗原的scFv抗体。
A semisynthetic antibody library composed of single a) chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons, After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones, The library was screened for binders to dinitrophenol, fluorescein isothiocyanate and 3-nitro-4-hydroxy-5-iodophenyl-acetic acid, scFv antibodies that specifically bound the antigen were obtained in each case.