Expression, purification, crystallization and preliminary X-ray crystallographic analysis of pantothenate kinase from Mycobacterium tuberculosis
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of pantothenate kinase from Mycobacterium tuberculosis
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DOI：
10.1107/s1744309104028040
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发表时间：
2005-01-01
影响因子：
0.9
通讯作者：
Vijayan, M
中科院分区：
文献类型：
--
作者：
Das, S;Kumar, P;Vijayan, M
泛酸激酶是细菌生命周期中必需的酶。催化泛酸（维生素B-5）磷酸化为4 '-磷酸泛酸，这是辅酶A生物合成途径的第一步。来自结核分枝杆菌（Mycobacterium tuberculosis）的酶（MW 35.7 kDa）已被克隆、表达、纯化并以两种不同的三角晶型结晶，两者均属于空间群P3（1）21。在室温下，在家用X射线源上，分别从晶胞参数a = B = 78.3，c = 115.45埃和a = B = 107.63，c = 89.85埃的晶体中收集分辨率为2.5埃（I型）和2.9埃（II型）的两个完整数据集。两种晶型的结构通过分子置换得到不对称单元中的一个亚基。
Pantothenate kinase is an essential enzyme in the bacterial life cycle. It catalyzes the phosphorylation of pantothenate ( vitamin B-5) to 4'-phosphopantothenate, the first step in the coenzyme A biosynthetic pathway. The enzyme from Mycobacterium tuberculosis, MW 35.7 kDa, has been cloned, expressed, purified and crystallized in two different trigonal crystal forms, both belonging to space group P3(1)21. Two complete data sets of resolution 2.5 angstrom (form I) and 2.9 angstrom (form II) from crystals with unit-cell parameters a = b = 78.3, c = 115.45 angstrom and a = b = 107.63, c = 89.85 angstrom, respectively, were collected at room temperature on a home X-ray source. Structures of both crystal forms were solved for one subunit in the asymmetric unit by molecular replacement.