ENHANCED PRODUCTION OF THE MINOR COMPONENTS OF GLIDOBACTINS IN POLYANGIUM-BRACHYSPORUM
ENHANCED PRODUCTION OF THE MINOR COMPONENTS OF GLIDOBACTINS IN POLYANGIUM-BRACHYSPORUM
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DOI：
10.7164/antibiotics.41.1358
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发表时间：
1988-10-01
影响因子：
3.3
通讯作者：
KAWAGUCHI, H
中科院分区：
文献类型：
--
作者：
NUMATA, K;MURAKAMI, T;KAWAGUCHI, H
短孢多角菌Polyangum brachysporum sp.11月ATCC 53080菌株产生一种新型的抗真菌和抗肿瘤的抗生素复合体--胶质结合素A、B和C，通过改进培养基能提高次要成分胶质结合素B和C的产量。在发酵培养基中添加富含不饱和C18脂肪酸的大豆油或玉米油，可以提高B组分和C组分的产量。通过添加富含油酸的油、橄榄油和吐温80(聚氧乙烯山梨醇单油酸酯)选择性地提高了C组分的产量。此外，棕榈油酸、亚油酸和油酸的前体分别允许A、B和C组分的直接生物合成。在初始添加3%的适量油的发酵条件下，B组分或C组分的产量最高。
Polyangium brachysporum sp. nov. strain ATCC 53080 produces a novel type of antifungal and antitumor antibiotic complex, glidobactins A, B and C. Enhanced production of minor components, glidobactins B and C, was achieved by medium modification. The addition of soybean oil or corn oil, which are rich in unsaturated C18 fatty acids, to the fermentation medium led to an increased production of components B and C. Productivity of component C was selectively enhanced by the addition of oleic acid-rich oils, olive oil and Tween 80 (polyoxyethylene sorbitan mono-oleate). Furthermore, precursing palmitoleate, linoleate and oleate permitted the direct biosynthesis of components A, B and C, respectively. The fermentation with 3% addition of an appropriate oil at initial time provided an optimal production of component B or C.