The Heat-Transfer Method: A Versatile Low-Cost, Label-Free, Fast, and User-Friendly Readout Platform for Biosensor Applications
The Heat-Transfer Method: A Versatile Low-Cost, Label-Free, Fast, and User-Friendly Readout Platform for Biosensor Applications
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DOI：
10.1021/am503667s
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发表时间：
2014-08-27
影响因子：
9.5
通讯作者：
Wagner, Patrick
中科院分区：
文献类型：
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作者：
van Grinsven, Bart;Eersels, Kasper;Wagner, Patrick
近年来，生物传感器在医学、生物学、药理学等各个科学领域发挥着越来越重要的作用，因此对快速有效的读出技术的需求也越来越大。在这篇聚焦应用的文章中，我们报道了最近开发的热传递方法(HTM)，并通过放大四个已建立的生物(模拟)传感器应用来说明该技术的使用：(I)DNA序列中的突变分析，(Ii)通过表面印迹聚合物识别癌细胞，(Iii)用分子印迹聚合物检测神经递质，以及(Iv)在脂囊泡层中的相变分析。该方法基于功能化固液界面热阻的变化。为此，该设备在该接口上施加温度梯度，并及时监测功能化芯片下方和上方的温度。通过将该温度梯度除以达到程序温度所需的功率，可以获得热传热阻。该技术的低成本、快速、无标签和用户友好的特性，加上高度的特异性、选择性和灵敏度，使HTM成为一种很有前途的传感器技术。
In recent years, biosensors have become increasingly important in various scientific domains including medicine, biology, and pharmacology, resulting in an increased demand for fast and effective readout techniques. In this Spotlight on Applications, we report on the recently developed heat-transfer method (HTM) and illustrate the use of the technique by zooming in on four established bio(mimetic) sensor applications: (i) mutation analysis in DNA sequences, (ii) cancer cell identification through surface-imprinted polymers, (iii) detection of neurotransmitters with molecularly imprinted polymers, and (iv) phase-transition analysis in lipid vesicle layers. The methodology is based on changes in heat-transfer resistance at a functionalized solid liquid interface. To this extent, the device applies a temperature gradient over this interface and monitors the temperature underneath and above the fimctionalized chip in time. The heat-transfer resistance can be obtained by dividing this temperature gradient by the power needed to achieve a programmed temperature. The low-cost, fast, label-free and user-friendly nature of the technology in combination with a high degree of specificity, selectivity, and sensitivity makes HTM a promising sensor technology.