Ontogenic Corticosteroidogenesis of the Domestic Fowl: Response of Isolated Adrenocortical Cells 1
Ontogenic Corticosteroidogenesis of the Domestic Fowl: Response of Isolated Adrenocortical Cells 1
复制标题
家禽的个体发育皮质类固醇发生：分离的肾上腺皮质细胞的反应 1
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
H. Weber
中科院分区：
文献类型：
--
作者：
R. Carsia;M. Morin;H. Rosen;H. Weber
用从鸡胚（18、19、20和21日龄）和孵化后的雄性和雌性鸟（1日龄、1周龄和3周龄）中分离的肾上腺皮质细胞研究了家养动物的个体发生肾上腺皮质功能。生产的主要皮质类固醇分泌的鸡肾上腺，皮质酮，皮质醇，和醛固酮，通过放射免疫测定法测定细胞孵育2小时后，有或无类固醇生成剂。接近孵化时，ACTH-（1-24）（ACTH）诱导的皮质类固醇产生的基础值和最大值稳定增加，在孵化后1天左右达到峰值（分别为胚胎18天时的5-18倍和3-9倍）。此后，皮质类固醇生产值稳步下降至3周postthatch。皮质酮占主导地位的年龄研究：最大促肾上腺皮质激素诱导的皮质酮生产平均52和115倍的醛固酮和皮质醇的生产值，分别。此外，最大促肾上腺皮质激素诱导的醛固酮生产大约是2.2倍以上的皮质醇生产的年龄研究，除了一个短暂的，不成比例的更大的醛固酮生产在1天后孵化。除了围孵化期和年龄相关的差异，在细胞皮质类固醇的生产，也有不同的细胞敏感性类固醇激素的药物，如在类固醇激素的药物的半最大类固醇浓度值（ED 50值）的差异所示。对ACTH的敏感性从胚胎期第18天到孵化后1天增加了2.7倍，然后稳步下降到孵化后3周。此外，对8-溴-cAMP（8-Br-cAMP）的敏感性在孵化后1天突然增加（近3倍），但此后保持不变。然而，直到孵化后3周才观察到细胞对25-羟基胆固醇敏感性的一致变化（敏感性增加3倍于胚胎生命第18天）。这些细胞敏感性的数据表明，有明显的发育和成熟的细胞位点的ACTH，8-Br-cAMP，和25-羟基胆固醇作用的改变。因此，在家禽从胚胎到胚后的过渡期，肾上腺皮质细胞类固醇生成能力和包括皮质类固醇生成途径的一些细胞位点的功能发生了变化。
Ontogenic adrenocortical function of the domestic was investigated using adrenocortical cells isolated from embryonic chicks (18, 19, 20, and 21 days old) and male and female posthatch birds (1 day, 1 week, and 3 weeks old). Production of the predominant corticosteroids secreted by the chicken adrenal gland, corticosterone, cortisol, and aldosterone, was measured by radioimmunoassay after 2-hr incubation of cells with or without steroidogenic agents. Approaching hatch, basal and maximal ACTH-(1-24) (ACTH)-induced corticosteroid production increased steadily and peaked around 1 day posthatch (5-18 times and 3-9 times, respectively, the production values at 18 days embryonic life). Thereafter, corticosteroid production values decreased steadily to 3 weeks posthatch. Corticosterone predominated over the ages studied: Maximal ACTH-induced corticosterone production averaged 52 and 115 times the production values of aldosterone and Cortisol, respectively. In addition, maximal ACTH-induced aldosterone production was roughly 2.2 times greater than Cortisol production over the ages studied except for a short-lived, disproportionately greater aldosterone production at 1 day posthatch. In addition to perihatch and age-related differences in cellular corticosteroid production, there were also differences in cellular sensitivity to steroidogenic agents as indicated by the differences in half-maximal steroidogenic concentration values (ED50 values) of the steroidogenic agents. Sensitivity to ACTH increased 2.7 times from Day 18 of embryonic life to 1 day posthatch and then decreased steadily to 3 weeks posthatch. In addition, sensitivity to 8-bromo-cAMP (8-Br-cAMP) increased abruptly at 1 day posthatch (nearly 3 times) but then remained constant thereafter. However, a consistent change in cellular sensitivity to 25-hydroxycholesterol was not observed until 3 weeks posthatch (an increase in sensitivity of 3 times that at Day 18 of embryonic life). These data of cellular sensitivity suggest that there were distinct developmental and maturational alterations in the cellular loci at which ACTH, 8-Br-cAMP, and 25-hydroxycholesterol acted. Thus, during the transition from embryonic to postembryonic life of the domestic fowl, there are alterations in adrenocortical cell steroidogenic capacity and in the function of some cellular loci comprising the corticosteroidogenic pathway.
妊娠晚期羔羊胎儿肾上腺对促肾上腺皮质激素（1-24）敏感性增加的体内证据。
影响因子：
4.8
作者：
Rose,JC;Meis,PJ;Urban,RR;GreissJr,FC
通讯作者：
GreissJr,FC