Facts about FACT and transcript elongation through chromatin
Facts about FACT and transcript elongation through chromatin
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DOI：
10.1016/j.gde.2004.02.004
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发表时间：
2004-04-01
影响因子：
4
通讯作者：
Reinberg, D
中科院分区：
文献类型：
--
作者：
Belotserkovskaya, R;Reinberg, D
染色质背景下转录伸长的调节是一个非常感兴趣的话题。尽管染色质在体外对PoIII机制的转录构成了障碍，但这个过程在体内是相当有效的。重要的是，作为这一过程的一部分，活跃转录的基因的染色质结构也会发生变化。通过遗传学、生物化学和靶向蛋白质组学的方法，已经确定了大量与转录本延长有关的因素。然而，这些因素的确切作用和作用机制仍然不清楚。在过去的一年里，随着FACT和Spt6在转录延伸中的作用的阐明，出现了重大的进展。这些因素通过破坏核小体结构的稳定性以及重组PoIII所穿越的核小体来促进PoIII通过染色质。
The regulation of transcription elongation within the context of chromatin is a topic of great interest. Even though chromatin presents a barrier to transcription by the PoIII machinery in vitro, this process is rather efficient in vivo. Importantly, the chromatin structure of the actively transcribed genes is altered as part of this process. A large number of factors implicated in the control of transcript elongation have been identified through genetics, biochemistry and targeted proteomics approaches. However the precise roles and mechanisms of action of these factors remain obscure. A significant advance came about this past year with the elucidation of the roles of FACT and Spt6 in transcription elongation. These factors facilitate PoIII passage through chromatin by destabilizing the nucleosome structure as well as reassemble nucleosomes traversed by PoIII.