Leukocyte Elastase Inhibitor A New Regulator of PARP-1
Leukocyte Elastase Inhibitor A New Regulator of PARP-1
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DOI：
10.1111/j.1749-6632.2009.04701.x
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Torriglia, Alicia
中科院分区：
文献类型：
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作者：
Lepretre, Chloe;Sidoli, Giuseppina;Torriglia, Alicia
聚（ADP-核糖）聚合酶-1（PARP-1）使用NAD（+）作为底物以形成ADP-核糖。在细胞凋亡期间，半胱天冬酶切割PARP-1以避免过度的NAD消耗。由于PARP-1是参与半胱天冬酶依赖性细胞凋亡的DNA酶活性的关键调节剂，因此需要其裂解来促进DNA降解。为了探索半胱天冬酶非依赖性细胞死亡的情况，我们研究了PARP-1对酸性核酸内切酶白细胞弹性蛋白酶抑制剂（LET）衍生的DNase II（L-DNase IT）的影响。我们首次发现PARP-I和LEI/L-DNase II之间存在关联。出乎意料的是，我们观察到LEI影响PARP-1的自动修饰。
Poly(ADP-ribose) polymerase-1 (PARP-1) uses NAD(+) as a substrate to form ADP-ribose. During apoptosis, caspases cleave PARP-I to avoid excessive NAD consumption. Because PARP-1 is a key regulator of the activity of DNases involved in caspase-dependent apoptosis, its cleavage is required to promote DNA degradation. To explore the situation in caspase-independent cell death, we investigated the effect of PARP-1 on the acid endonuclease leukocyte elastase inhibitor (LET)-derived DNase II (L-DNase IT). We found for the first time an association between PARP-I and LEI/L-DNase II. Unexpectedly, we observed that LEI influenced the automodification of PARP-1.