HYPOXANTHINE AND ADENOSINE IN MURINE OVARIAN FOLLICULAR-FLUID - CONCENTRATIONS AND ACTIVITY IN MAINTAINING OOCYTE MEIOTIC ARREST
HYPOXANTHINE AND ADENOSINE IN MURINE OVARIAN FOLLICULAR-FLUID - CONCENTRATIONS AND ACTIVITY IN MAINTAINING OOCYTE MEIOTIC ARREST
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DOI：
10.1095/biolreprod33.5.1041
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发表时间：
1985-12-01
影响因子：
3.6
通讯作者：
COLEMAN, DL
中科院分区：
文献类型：
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作者：
EPPIG, JJ;WARDBAILEY, PF;COLEMAN, DL
使用高效液相色谱法估计了三组小鼠卵巢滤泡液中次黄嘌呤和腺苷的浓度：（1）孕马血清促性腺激素（PMSG）致敏小鼠;（2）注射人绒毛膜促性腺激素（hCG）后2小时的PMSG致敏小鼠;（3）注射人绒毛膜促性腺激素（hCG）后5小时的PMSG致敏小鼠。第1组小鼠卵泡液中次黄嘌呤的浓度为2-4 mM，腺苷的浓度为0.35-0.70 mM。第2组小鼠卵泡液中这些嘌呤的浓度没有差异，其中成熟已用hCG诱导，但样品是在生殖囊泡破裂（GVBD）前采集的。因此，这些嘌呤浓度的降低似乎不会诱导GVBD。在已经发生GVBD的第3组小鼠的卵泡液中观察到次黄嘌呤和腺苷浓度显著降低。这种减少可能是卵泡液量增加的结果。腺苷有一个显着的，但短暂的，在维持卵丘细胞封闭和裸露的卵母细胞减数分裂停滞的影响，所有的卵母细胞经历了GVBD的100分钟孵育在1 mM腺苷。当GVBD评估后3小时的文化，浓度高达5 mM腺苷未能维持减数分裂停滞。相反，次黄嘌呤（2-5 mM）在维持卵丘细胞封闭和裸露卵母细胞减数分裂停滞方面具有剂量依赖性作用，持续长达24 h。卵丘细胞封闭的卵母细胞总是更敏感的次黄嘌呤比剥卵。腺苷和次黄嘌呤在维持减数分裂阻滞方面有很强的协同作用：4 mM次黄嘌呤和0.75 mM腺苷可使95%以上的卵母细胞维持减数分裂阻滞培养长达24 h。这种作用可通过撤回嘌呤而完全逆转。据推测，这些嘌呤的协同效应可能是通过促进环磷酸腺苷合成（腺苷）和防止其水解（次黄嘌呤）而产生的。
The concentrations of hypoxanthine and adenosine in ovarian follicular fluid were estimated, using high-performance liquid chromatography, for three groups of mice: (1) pregnant mare''s serum gonadotropin (PMSG)-primed mice; (2) PMSG-primed mice 2 h after injection with human chorionic gonadotropin (hCG); and (3) PMSG-primed mice 5 h after injection with hCG. The concentration of hypoxanthine in follicular fluid of Group 1 mice was 2-4 mM and of adenosine was 0.35-0.70 mM. There was no difference in the concentrations of these purines in the follicular fluid of Group 2 mice, in which maturation had been induced with hCG but the samples were taken just before germinal vesicle breakdown (GVBD). Therefore, a decrease in the concentrations of these purines does not appear to induce GVBD. A significant decrease in the concentrations of hypoxanthine and adenosine was observed in the follicular fluid of Group 3 mice in which GVBD had already occurred. This decrease was probably a result of an increase in follicular fluid volume. Adenosine had a significant, but transient, effect in maintaining both cumulus cell-enclosed and denuded oocytes in meiotic arrest; all oocytes had undergone GVBD by 100 min incubation in 1 mM adenosine. When GVBD was assessed after 3 h culture, concentrations up to 5 mM adenosine failed to maintain meiotic arrest. In contrast, hypoxanthine (2-5 mM) had a dose-dependent effect in maintaining both cumulus cell-enclosed and denuded oocytes in meiotic arrest that was sustained up to 24 h. Cumulus cell-enclosed oocytes were always more sensitive to hypoxanthine than were denuded oocytes. There was a strong synergistic effect of adenosine and hypoxanthine in maintaining meiotic arrest: 4 mM hypoxanthine and 0.75 mM adenosine maintained more than 95% of the oocytes in meiotic arrest for culture periods up to 24 h. This action was completely reversible by withdrawal of the purines. It is hypothesized that the synergistic effect of these purines may result both by promoting cyclic adenosine monophosphate synthesis (adenosine), and by preventing its hydrolysis (hypoxanthine).