Comparison of the accuracy of CapitalBio Mycobacterium real-time polymerase chain reaction and Xpert MTB/RIF for the diagnosis of tuberculous meningitis.
Comparison of the accuracy of CapitalBio Mycobacterium real-time polymerase chain reaction and Xpert MTB/RIF for the diagnosis of tuberculous meningitis.
复制标题
博奥分枝杆菌实时聚合酶链反应与Xpert MTB/RIF诊断结核性脑膜炎的准确性比较。
DOI：
10.1016/j.ijid.2020.12.044
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Jinpeng Huang
中科院分区：
文献类型：
--
作者：
Sun Lihua;Yao Liwei;Genlian Fu;Lin Lihua;Zhu Enlan;Jinpeng Huang
目的比较CapitalBioMycobacterium实时聚合酶链反应（PCR）检测试剂盒与标准Xpert MTB/RIF检测试剂盒诊断结核性脑膜炎（TBM）的效率。方法我们分析了2018年1月1日至2019年12月31日期间收集的163例疑似TBM患者的脑脊液（CSF）。对于这两种测试，我们确定了灵敏度，特异性，阳性预测值（PPV），阴性预测值（NPV）和曲线下面积（AUC）。接下来，我们比较了这两种技术的诊断准确性，使用临床诊断作为参考standard.ResultsThe的灵敏度，特异性，PPV，NPV，和AUC的CapitalBioMycobacteriumdetection测试分别为48.5%，100%，100%，29.6%，和0.74，当用于诊断TBM。相比之下，Xpert MTB/RIF检测试剂盒的返回值分别为47.0%、100%、100%、29.0%和0.74。我们的分析表明，CapitalBioMycobacteriumdetection测试和Xpert MTB/RIF检测的诊断准确性是非常相似的，这两个测试检测分枝杆菌的准确性是显着高于相关的抗酸staining.ConclusionsThe CapitalBioMycobacteriumreal-time PCR检测测试具有中等的敏感性和非常高的特异性，为TBM的结果是非常相似的Xpert MTB/RIF检测所产生的。我们建议将CapitalBio PCR检测作为结核病的初步筛查方法。
ObjectivesWe aimed to compare the efficiency of the CapitalBioMycobacteriumreal-time polymerase chain reaction (PCR) detection test with the standard Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis (TBM).MethodsWe analyzed cerebrospinal fluid (CSF) from 163 patients with suspected TBM that were collected between January 1, 2018, and December 31, 2019. For both tests, we determined the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the curve (AUC). Next, we compared the diagnostic accuracy of the two techniques using clinical diagnosis as a reference standard.ResultsThe sensitivity, specificity, PPV, NPV, and AUC, of the CapitalBioMycobacteriumdetection test were 48.5%, 100%, 100%, 29.6%, and 0.74, respectively, when used for the diagnosis of TBM. In comparison, the Xpert MTB/RIF assay returned values of 47.0%, 100%, 100%, 29.0%, and 0.74, respectively. Our analysis showed that the diagnostic accuracies of the CapitalBioMycobacteriumdetection test and the Xpert MTB/RIF assay were very similar; the accuracy of both tests for detecting mycobacteria was significantly higher than that associated with acidfast staining.ConclusionsThe CapitalBioMycobacteriumreal-time PCR detection test has moderate sensitivity and very high specificity for TBM; results are very similar to those generated by the Xpert MTB/RIF assay. We recommend that the CapitalBio PCR test should be used as an initial screening method for TB.
影响因子：
11.8
作者：
Bahr, Nathan C.;Marais, Suzaan;Boulware, David R.
通讯作者：
Boulware, David R.