Quantatitive assessment of enzyme immobilization capacity in porous silicon
Quantatitive assessment of enzyme immobilization capacity in porous silicon
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DOI：
10.1021/ac0488208
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发表时间：
2004-12-01
影响因子：
7.4
通讯作者：
Miller, BL
中科院分区：
文献类型：
--
作者：
DeLouise, LA;Miller, BL
固定化酶系统在从生物传感到手性产物的工业规模生物催化合成的广泛应用中是重要的。我们证明了系统地改变和定量评估的固定能力的多孔硅薄膜的酶谷胱甘肽-S-转移酶的多孔硅基质的一个简单的几何模型预测的方式。我们发现，固定能力定量相关的设备厚度的系统变化。这些结果是显着的，因为，尽管多孔硅的形态和表面积可以变化的范围很宽，很少有人尝试系统地表征表面结合能力。我们的研究结果表明，多孔硅可以是一个理想的矩阵，其中固定的生物材料的可预测的数量是期望的。
Immobilized enzyme systems are important in a broad range of applications, from biological sensing to the industrial-scale biocatalytic synthesis of chiral products. We demonstrate the ability to systematically vary and quantitatively assess the immobilization capacity of porous silicon thin films for the enzyme glutathione-S-transferase in a manner predicted by a simple geometric model of the porous silicon matrix. We find that the immobilization capacity quantatitively correlates with systematic changes in the device thickness. These results are significant since, despite the wide range over which porous silicon morphology and surface area can be varied, few attempts have been made to systematically characterize surface binding capacity. Our findings suggest that porous silicon can be an ideal matrix, where immobilization of a predictable quantity of biological material is desired.