Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection.
Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection.
复制标题
DOI：
10.1016/j.fsi.2014.11.018
复制
发表时间：
2015-03
影响因子：
4.7
通讯作者：
Xiang Zhang;Shaolin Wang;Songlin Chen;Ya-dong Chen;Yang Liu;C. Shao;Qilong Wang;Yang Lu;Guangye Gong;S. Ding;Z. Sha
中科院分区：
文献类型：
--
作者：
Xiang Zhang;Shaolin Wang;Songlin Chen;Ya-dong Chen;Yang Liu;C. Shao;Qilong Wang;Yang Lu;Guangye Gong;S. Ding;Z. Sha
半滑舌鳎（Cynoglossus semilaevis）是中国北方最有价值的海洋水生物种之一。鉴于水产养殖业的快速发展，C. 半滑鼠感染了致病细菌鳗弧菌。因此，了解C的机制是必不可少且紧迫的。 半左旋宿主防御V。 鳗鱼感染。在本研究中，在转录组水平上对V进行了广泛的分析。 进行了舌鳎鳗鱼病检查。三个文库（HOSG、NOSG 和 CG）中的 75,884,572 个干净读数总共生成了 94,716 个高质量重叠群。与NR蛋白质数据库和NT核苷酸数据库SwissProt相比，鉴定出22,746个unigenes。鉴定出在所有三个文库中至少一对比较中表现出差异表达的 954 个基因。这些基因的 GO 富集揭示了基因对生物刺激的反应、免疫系统调节以及免疫反应和细胞因子的产生。此外，补体和凝血级联以及霍乱弧菌感染途径等途径在病原体防御方面也得到了丰富。此外，在舌鳎中检测到13,428个SSR和118,239个SNP，为今后的遗传变异和标记辅助选择提供了进一步的支持。总之，本研究确定了一些假定的免疫途径和候选基因，值得在治疗方案开发的背景下进一步研究，并为选择念珠菌耐药系奠定了基础。 半左翼反对V。 鳗鱼。
Half-smooth tongue sole (Cynoglossus semilaevis) is one of the most valuable marine aquatic species in Northern China. Given to the rapid development of aquaculture industry, theC. semilaeviswas subjected to disease-causing bacteriaVibrio anguillarum. It therefore is indispensable and urgent to understand the mechanism ofC. semilaevishost defense againstV. anguillaruminfection. In the present study, the extensively analysis at the transcriptome level forV. Anguillarumdisease in tongue sole was carried out. In total, 94,716 high quality contigs were generated from 75,884,572 clean reads in three libraries (HOSG, NOSG, and CG). 22,746 unigenes were identified when compared with SwissProt, an NR protein database and NT nucleotide database. 954 genes exhibiting the differentially expression at least one pair of comparison in all three libraries were identified. GO enrichment for these genes revealed gene response to biotic stimulus, immune system regulation, and immune response and cytokine production. Further, the pathways such as complement and coagulation cascades andVibrio choleraeinfection pathways were enriched in defensing of pathogen. Besides, 13,428 SSRs and 118,239 SNPs were detected in tongue sole, providing further support for genetic variation and marker-assisted selection in future. In summary, this study identifies several putative immune pathways and candidate genes deserving further investigation in the context of development of therapeutic regimens and lays the foundation for selecting resistant lines ofC. semilaevisagainstV. anguillarum.