How to stabilize phospholipid liposomes (using nanoparticles)
How to stabilize phospholipid liposomes (using nanoparticles)
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DOI：
10.1021/nl052455y
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发表时间：
2006-04-01
期刊：
影响因子：
10.8
通讯作者：
Granick, S
中科院分区：
文献类型：
--
作者：
Zhang, LF;Granick, S
将磷脂脂质体与带电纳米颗粒混合并使用超声处理以低体积分数将它们混合的简单策略产生彼此排斥且不融合的颗粒稳定的脂质体。随后，体积分数可以可逆地升高到高达约50%，仍然没有熔合。在脂质体寿命研究中，我们验证了体积分数高达16%的颗粒稳定脂质体混悬液的稳定性，最长可持续50天，这是研究的最长时间。荧光染料包封在颗粒稳定的脂质体内，没有泄漏。尽管这些颗粒稳定的脂质体对融合是稳定的，但约75%的脂质体外表面保持未被占据。这为在各种应用中使用颗粒稳定的脂质体打开了大门。
The simple strategy of mixing phospholipid liposomes with charged nanoparticles and using sonication to mix them at low volume fraction produces particle-stabilized liposomes that repel one another and do not fuse. Subsequently, the volume fraction can be raised as high as approximate to 50%, reversibly, still without fusion. In studies of liposome longevity, we verified the stability of particle-stabilized liposome suspensions with volume fraction up to 16% for up to 50 days, the longest period investigated. Fluorescent dyes were encapsulated within the particle-stabilized liposomes, without leakage. Although these particle-stabilized liposomes were stable against fusion, approximate to 75% of the outer liposome surface remained unoccupied. This opens the door to using particle-stabilized liposomes in various applications.