MCM4 expression in esophageal cancer from southern China and its clinical significance
MCM4 expression in esophageal cancer from southern China and its clinical significance
复制标题
DOI：
10.1007/s00432-005-0011-6
复制
发表时间：
2005-10-01
影响因子：
3.6
通讯作者：
Fang, Y
中科院分区：
文献类型：
--
作者：
Huang, XP;Rong, TH;Fang, Y
目的和实验设计：MCM 4是微小染色体维持蛋白家族的成员。MCM 2 -7蛋白在真核生物DNA复制中起重要作用，并已被鉴定为DNA复制许可因子的组分。到目前为止，还没有关于MCM 4在食管癌中的研究报道。本研究通过RT-PCR方法检测MCM 4在中国南方食管癌组织中的表达情况，从而揭示MCM 4与食管癌的关系。结果如下：65%（39/60）的食管癌组织中MCM 4表达增高，而正常食管上皮组织中大多数不表达或低表达MCM 4。60例中20例（33%）癌旁上皮细胞中MCM 4表达增强。食管癌组织中MCM 4的表达明显高于癌旁组织（χ 2值为12.037，P < 0.001）。T3期和T1期胃癌中MCM 4的表达差异有统计学意义（χ 2 =4.038，P < 0.05）。结论：食管癌组织中MCM 4的高表达可能与食管癌的病理分期有关。MCM 4的改变可能与食管癌发生的早期事件有关。MCM 4可能是食管癌发生发展的一个有价值的分子标志物。
Purpose and Experimental Design: MCM4 is a member of Minichromosome maintenance protein family. MCM2-7 proteins play an essential role in eukaryotic DNA replication and have been identified as components of DNA replication licensing factors. So far, no research on MCM4 has been reported in esophageal cancer. In this study, we detected via RT-PCR the expression status of MCM4 in esophageal cancer from southern China and therefore disclose the relationship between MCM4 and esophageal cancer. Results: 65% (39/60) cases showed increased expression of MCM4 in the carcinomas when compared with normal esophageal epithelia in which no or low MCM4 expression was detected in most cases. Twenty of sixty cases (33%) showed increased expression of MCM4 in the adjacent epithelia. Furthermore, MCM4 expression in esophageal carcinomas was significantly higher than the one in the adjacent epithelia (chi square value is 12.037, P < 0.001). Significant difference for the expression status of MCM4 was found between the patients with histopathological stage T3 and stage T1 (chi square value=4.038, P < 0.05). Conclusions: The increased expression of MCM4 might be associated with pathological staging of esophageal cancer. The alterations of MCM4 are possibly related to the earlier event of esophageal carcinogenesis. MCM4 is probably a valuable molecular marker involved in the development and/or genesis of esophageal cancer.