Circular dichroism studies of cobalt substituted lentil lectin.
Circular dichroism studies of cobalt substituted lentil lectin.
复制标题
钴取代扁豆凝集素的圆二色性研究。
DOI：
10.1016/0006-291x(86)90930-7
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Brewer,CF
中科院分区：
文献类型：
--
作者：
Stafford,E;Behnke,WD;Bhattacharyya,L;Brewer,CF
已经开发了一种最近的方法来实现在小扁豆凝集素中Mn 2+位点处的金属离子取代（Bhattacharyya等人（1984）Biochem.Biophys.Biophys.通信资源124，857-862）。我们在这里报告的钴取代扁豆凝集素的制备，含有Co 2+在S1网站和Ca 2+在S2网站。该钴衍生物具有完全的糖结合活性，可用于光谱研究。给出了该衍生物的近紫外和可见CD光谱，并将其光谱性质与伴刀豆球蛋白A的各种钴配合物进行了比较。
A recent method has been developed to effect metal ion substitution at the Mn2+site in the lentil lectin (Bhattacharyya et al. (1984) Biochem. Biophys. Res. Commun. 124, 857–862). We report here the preparation of cobalt substituted lentil lectin, containing Co2+at the S1 site and Ca2+at the S2 site. The cobalt derivative possesses full saccharide binding activity and can be used for spectroscopic studies. The near UV and visible CD spectra of the derivative are shown, and its spectral properties are compared with various cobalt complexes of concanavalin A.