An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.
An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.
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DOI：
10.1083/jcb.105.1.41
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发表时间：
1987-07
期刊：
影响因子：
--
通讯作者：
Fordham M
中科院分区：
文献类型：
--
作者：
White JG;Amos WB;Fordham M
扫描共聚焦显微镜提供了比传统成像更好的对焦外噪声的抑制和更高的分辨率。在这样的显微镜中，成像透镜和聚光透镜是相同的，并且是共焦的。当使用表观照明时，这两个透镜被一个透镜取代，使得共焦成像特别适用于入射光学显微镜。我们描述了我们用共焦系统获得的结果，在该系统中，扫描是通过移动光束而不是工作台来执行的。该系统比扫描台式显微镜速度快得多，而且使用方便。我们发现，共聚焦成像大大增强了用荧光观察的生物结构的图像。这种改进使得可以光学切片较厚的试件，而内部切片的图像质量几乎不会降低。
Scanning confocal microscopes offer improved rejection of out-of-focus noise and greater resolution than conventional imaging. In such a microscope, the imaging and condenser lenses are identical and confocal. These two lenses are replaced by a single lens when epi- illumination is used, making confocal imaging particularly applicable to incident light microscopy. We describe the results we have obtained with a confocal system in which scanning is performed by moving the light beam, rather than the stage. This system is considerably faster than the scanned stage microscope and is easy to use. We have found that confocal imaging gives greatly enhanced images of biological structures viewed with epifluorescence. The improvements are such that it is possible to optically section thick specimens with little degradation in the image quality of interior sections.