Morphological characterization of pecteneal hyalocytes in the developing quail retina
Morphological characterization of pecteneal hyalocytes in the developing quail retina
复制标题
DOI：
10.1111/j.1469-7580.2009.01117.x
复制
发表时间：
2009-09-01
影响因子：
2.4
通讯作者：
Ruberte, Jesus
中科院分区：
文献类型：
--
作者：
Llombart, Cristina;Nacher, Victor;Ruberte, Jesus
玻璃体的周边含有一群称为透明细胞的细胞。尽管存在超过一个世纪的出版物致力于pecten oculi，一个回旋的线圈的血管，似乎是主要来源的营养，鸟类无血管视网膜，很少的信息可以找到有关pecteneal透明细胞。这些细胞位于内界膜上，与回旋血管关系密切。为了描述鹌鹑眼透明细胞的起源和巨噬细胞的活性，我们用组织化学和免疫组织化学方法分析了鹌鹑眼发育的两个不同阶段。果胶透明细胞表达QH 1表位和cKit，证实这些细胞属于造血系统。它们还表达波形蛋白，一种存在于间充质来源的细胞中的中间丝蛋白，对于完全活性的巨噬细胞的分化非常重要。然而，与猪透明细胞中所述类似，胸鳍透明细胞表达胶质细胞酸性蛋白，这是一种公认的神经胶质标志物。果胶透明细胞不表达其他神经胶质标记物，如谷氨酰胺合成酶或S100。酸性磷酸酶被激活，Lep 100被发现在次级溶酶体中，证实在眼发育过程中的栉孔透明细胞的吞噬活性。胸腺透明细胞与RCA-Ⅰ、WFA、WGA、PNA、SNA、莱亚和SBA凝集素反应强烈，而胸腺和法氏囊巨噬细胞不与PNA、SNA和莱亚凝集素结合。有趣的是，WGA凝集素与所有种类的禽类巨噬细胞反应，包括栉孔透明细胞，可能反映了WGA与吞噬和内吞途径的组分的特异性结合。总之，栉孔透明细胞是一种特殊亚型的血液传播的巨噬细胞，表达的标志物不专门与造血系统。
The periphery of the vitreous body contains a population of cells termed hyalocytes. Despite the existence for more than one century of publications devoted to the pecten oculi, a convoluted coil of blood vessels that seems to be the primary source of nutrients for the avian avascular retina, little information can be found concerning the pecteneal hyalocytes. These cells are situated on the inner limiting membrane in close relationship with the convolute blood vessels. To characterize the origin and macrophagic activity of pecteneal hyalocytes, we have analysed two different stages of quail eye development using histochemistry and immunohistochemistry. Pecteneal hyalocytes express the QH1 epitope and cKit, confirming that these cells belong to the haematopoietic system. They also express vimentin, an intermediate filament protein present in cells of mesenchymal origin and very important for differentiation of fully active macrophages. However, similarly as described in porcine hyalocytes, pecteneal hyalocytes express the glial fibrillary acidic protein, a recognized neuroglial marker. Pecteneal hyalocytes did not express other neuroglial markers, such as glutamine synthetase or S100. Acidic phosphatase was activated and Lep100 was found in secondary lysosomes, confirming phagocytic activity of pecteneal hyalocytes during ocular development. Pecteneal hyalocytes strongly react with RCA-I, WFA, WGA, PNA, SNA, LEA and SBA lectins, whereas other avian macrophages from thymus and the bursa of Fabricius did not bind PNA, SNA and LEA lectins. Interestingly, WGA lectin reacts with all kinds of avian macrophages, including pecteneal hyalocytes, probably reflecting the specific binding of WGA to components of the phagocytic and endocytic pathways. In conclusion, pecteneal hyalocytes are a special subtype of blood-borne macrophages that express markers not specifically associated with the haematopoietic system.