Heat treatment improves the exaggerated exercise pressor reflex in rats with femoral artery occlusion via a reduction in the activity of the P2X receptor pathway.
Heat treatment improves the exaggerated exercise pressor reflex in rats with femoral artery occlusion via a reduction in the activity of the P2X receptor pathway.
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DOI：
10.1113/jp279230
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发表时间：
2020-04
期刊：
影响因子：
--
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Qin L;Li Q;Li J
本研究旨在观察热处理（HT）是否对外周动脉疾病（PAD）大鼠的过度运动加压反射有有益作用。我们进一步确定了温度敏感性P2 X受体是否参与HT的作用。用静态肌肉收缩和α，β-亚甲基ATP（αβ-meATP，一种P2 X激动剂）检测升压反应。Western blot检测背根神经节（DRG）P2 X3蛋白表达，全细胞膜片钳技术检测P2 X电流。PAD大鼠的基础肌温（Tm）低于对照组。Tm增加约1.5°C，并保持这种增加30分钟。该HT方案每天进行两次，连续三天。在PAD大鼠中观察到更大的血压（BP）对收缩的反应。HT可减弱PAD大鼠血压反应的放大作用。HT还能减弱动脉注射αβ-meATP引起的PAD大鼠血压反应的增强。此外，HT还能减弱PAD大鼠DRG神经元P2 X 3的上调，增加P2 X电流。总之，先前的热暴露在修改PAD中的运动加压反射的放大中起抑制作用，并且P2 X受体通路的活性的降低可能是导致HT的有益作用的机制的一部分。
The current study was performed to examine if heat treatment (HT) has beneficial effects on the exaggerated exercise pressor reflex in rats with peripheral artery disease (PAD). We further determined if the temperature-sensitive P2X receptor is involved in the effects of HT. The pressor response to static muscle contraction and α,β-methylene ATP (αβ-me ATP, a P2Xagonist) was examined. Western blot analysis was used to determine the protein levels of P2X3 in the dorsal root ganglion (DRG), and the whole cell patch clamp was used to examine the amplitude of P2X currents in the DRG neurons. The basal muscle temperature (Tm) was lower in PAD rats than in control rats. Tm was increased by ~1.5°C and this increase was maintained for 30 min. This HT protocol was performed tweice daily for three continuous days. A greater blood pressure (BP) response to contraction was observed in PAD rats. HT attenuated the amplification of the BP response in PAD rats. HT also attenuated the enhancement of the BP response induced by the arterial injection of αβ-me ATP in PAD rats. In addition, HT attenuated the upregulation of the P2X3 and increased P2X currents in the DRG neurons of PAD rats. In conclusion, previous heat exposure plays an inhibitory role in modifying the exaggeration of the exercise pressor reflex in PAD and a reduction of the activity of the P2X receptor pathway is probably a part of mechanisms leading to the beneficial effects of HT.