MAPPING OF GLUCOCORTICOID RECEPTOR IMMUNOREACTIVE NEURONS IN THE RAT TELENCEPHALON AND DIENCEPHALON USING A MONOCLONAL-ANTIBODY AGAINST RAT-LIVER GLUCOCORTICOID RECEPTOR
MAPPING OF GLUCOCORTICOID RECEPTOR IMMUNOREACTIVE NEURONS IN THE RAT TELENCEPHALON AND DIENCEPHALON USING A MONOCLONAL-ANTIBODY AGAINST RAT-LIVER GLUCOCORTICOID RECEPTOR
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DOI：
10.1210/endo-117-5-1803
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发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
GUSTAFSSON, JA
中科院分区：
文献类型：
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作者：
FUXE, K;WIKSTROM, AC;GUSTAFSSON, JA
通过对大鼠肝糖皮质激素受体（GR）的单克隆抗体与间接免疫过氧化物酶技术相结合的装置，它已经有可能证明GR免疫反应神经和胶质细胞核遍布的tel和间脑的雄性大鼠。强GR-免疫反应阳性神经细胞核只存在于室旁核的小细胞部分，在下丘脑室周核的前部，在下丘脑内侧基底核的腹侧部分，并在海马结构的CA 1和CA 2亚区。在下丘脑室前核的GR免疫反应区和CRF免疫反应区之间存在大量的重叠。中到高密度的中度GR免疫反应神经细胞核存在于整个皮质半球。中等密度的中度GR免疫反应神经细胞被证明在许多丘脑核和中央杏仁核。肾上腺切除后GR免疫反应主要位于心包。肾上腺切除雄性大鼠急性皮质酮治疗后，GR免疫反应性再次主要表现在神经细胞核中，表明皮质酮可以将GR从细胞质转运到细胞核。提示下丘脑GR不仅参与调节CRF的分泌，而且还参与调节其他垂体前叶激素如TRH和生长抑素的分泌。
By means of a monoclonal antibody against the rat liver glucocorticoid receptor (GR) in combination with the indirect immunoperoxidase technique it has been possible to demonstrate GR-immunoreactive nerve and glial cell nuclei all over the tel- and diencephalon of the male rat. Strongly GR-immunoreactive nerve cell nuclei were only present in the parvocellular part of the paraventricular hypothalamic nucleus, in the anterior periventricular hypothalamic nucleus, in the ventral part of the mediobasal hypothalamus, and in the CA1 and CA2 subregion of the hippocampal formation. Within the praventricular hypothalamic nucleus a substantial overlap exists between the GR-immunoreactive area and the CRF-immunoreactive area. Medium to high densities of moderately GR-immunoreactive nerve cell nuclei were present all over the cortical hemispheres. Medium densities of moderately GR-immunoreactive nerve cells were demonstrated in many thalamic nuclei and in the central amygdaloid nucleus. After adrenalectomy the GR immunoreactivity was predominantly located in the pericaryon. Upon acute coticosterone treatment of adrenalectomized male rats, the GR immunoreactivity was again mainly demonstrated in the nerve cell nuclei indicating that corticosterone can translocate GR from the cytoplasm to the cell nuclei. It is suggested that the hypothalamic GR may be involved in the regulation of especially CRF secretion but also in the secretion of other anterior pituitary hormones such as TRH and somatostatin.