Repair of intra-thoracic autonomic nerves using chitosan tubes
Repair of intra-thoracic autonomic nerves using chitosan tubes
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DOI：
10.1510/icvts.2009.227744
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发表时间：
2010-04-01
影响因子：
--
通讯作者：
Watanabe, Go
中科院分区：
文献类型：
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作者：
Matsumoto, Isao;Kaneko, Mami;Watanabe, Go
我们研究了壳聚糖纳米/微纤维网状管(C管)在损伤的胸部自主神经再生中的安全性和有效性。在6只狗身上进行了动物实验。切除右侧胸交感神经(实验1，n=3)和膈神经(实验2，n=3)，C管缝合。术后，实验1观察霍纳综合征。在实验2中，通过胸部X光成像评估隔膜的活动度。在这两个实验中，对神经再生进行了病理学评估。在整个观察期内，所有六只狗都存活下来，没有出现并发症。在实验1中，交感神经在3个月后开始在C管内再生。术后7个月和12个月，交感神经连接。虽然三只狗在手术后都患有右霍纳氏综合征，但在12个月后病情有所改善。在实验2中，在12个月时，三只狗中的两只连接了膈神经。在X光成像中，虽然三只狗都有横隔膜突出，但右横隔膜随着呼吸的变化而移动，在这些狗身上，膈神经再生了。C-管可以安全地用于促进受损交感神经和膈神经的再生和恢复其丧失的功能。(C)2010年，由欧洲心胸外科协会出版。版权所有。
We investigated the safety and usefulness of chitosan nano/microfiber mesh tubes (C-tubes) in regenerating damaged thoracic autonomic nerves. Animal experiments were performed in six dogs. The right thoracic sympathetic nerve (Experiment 1, n=3) and phrenic nerve (Experiment 2, n=3) were resected and sutured with C-tubes. After surgery, in Experiment 1, Horner's syndrome was observed. In Experiment 2, mobility of diaphragm was assessed by chest X-ray imaging. Nerve regeneration was assessed pathologically in both experiments. All six dogs survived without complication throughout the observational period. In Experiment 1, sympathetic nerve began to regenerate inside the C-tube at three months. At 7 and 12 months after surgery, the sympathetic nerves were connected. Though all three dogs had right Horner's syndrome after surgery, it improved at 12 months. In Experiment 2, at 12 months, the phrenic nerves were connected in two of the three dogs. In X-ray imaging, though all three dogs had eventration of the diaphragm, the right diaphragm moved in response to breathing in the dogs in which phrenic nerve regenerated. C-tubes can be safely used to facilitate the regeneration of damaged sympathetic and phrenic nerves and the restoration of their lost functions. (c) 2010 Published by European Association for Cardio-Thoracic Surgery. All rights reserved.