Neonatal Fc Receptor-Mediated IgG Transport Across Porcine Intestinal Epithelial Cells: Potentially Provide the Mucosal Protection
Neonatal Fc Receptor-Mediated IgG Transport Across Porcine Intestinal Epithelial Cells: Potentially Provide the Mucosal Protection
复制标题
新生儿 Fc 受体介导的 IgG 跨猪肠上皮细胞转运：可能提供粘膜保护
DOI：
10.1089/dna.2015.3165
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发表时间：
2016-06-01
影响因子：
3.1
通讯作者：
Li, Zili
中科院分区：
文献类型：
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作者：
Guo, Jinyue;Li, Fei;Li, Zili
众所周知，仔猪可以通过肠道吸收初乳 IgG 进入新生儿血液，并且在猪肠道粘膜分泌物中也发现了一定水平的 IgG。然而，对于母体 IgG 如何穿过肠道屏障以及 IgG 如何进入肠道内腔，人们知之甚少。在本研究中，我们证明猪新生Fc受体（pFcRn）在正常猪肠上皮细胞（IPEC-J2）模型以及肾细胞（PK-15）中表达，并且pFcRn主要分布在极化的IPEC-J2细胞的顶端侧。分析该基因的系统发育相关性，我们发现猪和人类新生儿 Fc 受体 (FcRn) 氨基酸序列比啮齿类动物更接近。我们还表明，pFcRn 介导跨极化 IPEC-J2 细胞的双向 IgG 转运，并以 pH 依赖性方式与 IgG 结合。此外，通过 50% 组织培养感染剂量 (TCID50) 测定，pFcRn 转胞病毒特异性 IgG 降低了管腔方向的传染性胃肠炎病毒 (TGEV) 产量。我们的结果表明，pFcRn 依赖性双向 IgG 跨肠上皮转运在生命早期体液免疫的获得和粘膜表面的宿主防御中发挥着关键作用。
It has been well characterized that piglets can absorb colostrum IgG across the intestine to neonatal bloodstream and a certain level of IgG has been found in the mucosal secretions of the porcine intestinal tract. However, little is known about how the maternal IgG transport across the intestinal barrier and how IgG enter the lumen of intestinal tract. In this study, we demonstrated that the porcine neonatal Fc receptor (pFcRn) was expressed in a model of normal porcine intestinal epithelial cells (IPEC-J2) as well as in kidney cells (PK-15), and pFcRn was mainly distributed in the apical side of the polarized IPEC-J2 cells. Analyzing the phylogenetic relatedness of this gene we found that swine and human neonatal Fc receptor (FcRn) amino acid sequence are closer than rodents. We also showed that pFcRn mediated bidirectional IgG transport across polarized IPEC-J2 cells and bound to IgG in a pH-dependent manner. Furthermore, pFcRn-transcytosed viral-specific IgG reduced the transmissible gastroenteritis virus (TGEV) yield from the luminal direction by a 50% tissue culture infective dose (TCID50) assay. Our results indicate that pFcRn-dependent bidirectional IgG transport across the intestinal epithelium plays critical role in the acquisition of humoral immunity in early life and in host defense at mucosal surfaces.