Isolation of biologically active constituents from Moringa peregrina (Forssk.) Fiori. (family: Moringaceae) growing in Egypt.
Isolation of biologically active constituents from Moringa peregrina (Forssk.) Fiori. (family: Moringaceae) growing in Egypt.
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DOI：
10.4103/0973-1296.80667
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发表时间：
2011-04
影响因子：
0.7
通讯作者：
Kamel GM
中科院分区：
文献类型：
--
作者：
El-Alfy TS;Ezzat SM;Hegazy AK;Amer AM;Kamel GM
辣木是一种生长在埃及东部沙漠山区的野生植物。虽然这种植物原产于埃及，但没有对其化学成分和生物活性进行详细研究。用不同的硅胶柱和葡聚糖柱对植物风干部位的乙醇提取物进行分离纯化，分离出主要化合物，并对其抗癌活性进行了测试。采用水提物、醇提物及其不同组分对链霉菌素诱导的糖尿病大鼠的降糖作用进行了研究。通过对洋槐地面部位乙醇提取物不同馏分的研究，分别得到首次从该植物中分离得到的乙酸芦皮醇(1)、β-amyrin(2)、α-amyrin(3)、β-谷甾醇(4)、β-谷甾醇-3- o -葡萄糖苷(5)、芹菜素(6)、鼠李素(7)、新绿原酸（10）、鼠李苷-3- o - rutino苷（12）和6-甲氧基-acacetin-8- c -β-葡萄糖苷（13）。化合物（13）为首次从辣木属植物中分离得到。此外，还分离到了槲皮素(8)、金菊素-7- o -鼠李糖苷(9)和槲皮素-3- o -芦丁苷（11）。通过光谱数据（紫外、质谱、红外、1H、1H -1H COSY和13C-NMR）进行鉴定。主要分离的化合物被发现对乳腺癌（mcf7）和结肠癌（HCT 116）细胞系具有有价值的细胞毒活性，其活性与参比药阿霉素相当。另一方面，水提物和醇提物以及正己烷部分对链霉菌素诱导的糖尿病大鼠具有有效的降糖作用。埃及植物M. peregrina含有丰富的生物活性成分，显示出强大的细胞毒活性，其乙醇提取物具有显着的降糖作用。
Moringa peregrina is a wild plant that grown in the eastern desert mountains in Egypt. Although, this plant is native to Egypt, no details studies were traced on its chemical composition and biological activity. The different fractions of the ethanolic extract of the dried aerial parts of the plants were subjected to fractionation and purification on various silica and sephadex columns for the isolation of the major compounds which were tested for there anticancer activity. The aqueous and ethanolic extract as well as its different fractions were tested for antihyperglycemic effect on Streptozitocin-induced diabetes in rats. Investigation of the different fractions of the ethanolic extract of the aerial parts of M. peregrina yielded lupeol acetate (1), β-amyrin (2), α-amyrin (3), β-sitosterol (4), β-sitosterol-3-O-glucoside (5), apigenin (6), rhamnetin (7), neochlorogenic acid (10), rhamnetin-3-O-rutinoside (12), and 6-methoxy-acacetin-8-C-β-glucoside (13) which were isolated for the first time from the plant. Compound (13) was isolated for the first time from genus Moringa. In addition, quercetin (8), chryseriol-7-O-rhamnoside (9) and quercetin-3-O-rutinoside (11) were also isolated. Identification has been established by spectral data (UV, MS, IR, 1H, 1H -1H COSY, and 13C-NMR). The major isolated compounds were found to have valuable cytotoxic activities against breast (MCF 7) and colon (HCT 116) cancer cell lines and their activities were comparable to the reference drug doxorubicin. On the other hand, the aqueous and ethanolic extracts as well as the n-hexane fraction were found to have potent antihyperglycemic effect on Streptozitocin-induced diabetes in rats. The Egyptian plant M. peregrina is rich in biologically active ingredients which showed potent cytotoxic activity and also its ethanolic extraxt exert a significant antihyperglycemic effect.