Investigation and risk assessment of dibutyl phthalate in a typical region by a high-throughput dual-signal immunoassay
Investigation and risk assessment of dibutyl phthalate in a typical region by a high-throughput dual-signal immunoassay
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高通量双信号免疫分析典型区域邻苯二甲酸二丁酯的调查及风险评估
DOI：
10.1016/j.jhazmat.2021.127991
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发表时间：
2021
影响因子：
13.6
通讯作者：
Zhen Zhang
中科院分区：
文献类型：
--
作者：
Dinghui Xiong;Nuanfei Zhu;Fang Zhu;Salome Yakubu;Jungang Lv;Jingfu Liu;Zhen Zhang
采用超灵敏双信号免疫分析法对江苏省镇江市邻苯二甲酸二丁酯（DBP）进行了系统调查和风险评价。在本研究中，合成C-dots@H-MnO2nanohybrid并在二抗上标记以产生荧光和比色信号。由于碳点（C-dots）的高效催化和空心氧化锰（H-MnO2）的高C-dots负载，产生了优异的灵敏度和低检出限（分别为0.243和0.692 μg/L）。基于所提出的方法，对25种水和119种饮料样品进行了调查。所有水样和65.5%的饮料样品中均检测到DBP，浓度范围分别为16.5 ~ 32.1 μg/L和0 ~ 553 μg/L。水体中平均浓度（22.9 μg/L）较实验室2016年检测到的43.5 μg/L有明显下降。对于饮料，通过测量咖啡中的浓度也观察到类似的现象。结果表明，人类活动对当地水生生态系统造成了严重的污染和高风险。另一方面，健康风险评估结果表明，饮料中的DBP可能不会对当地居民造成明显的副作用。
The systematic investigation and risk assessment of dibutyl phthalate (DBP) were performed using an ultrasensitive dual-signal immunoassay in Zhenjiang, Jiangsu Province. In this study, C-dots@H-MnO2nanohybrid were synthesized and labelled on the secondary antibody to generate fluorometric and colorimetric signals. Attributed to the efficient catalysis of carbon dots (C-dots) and the high C-dots loading of hollow manganese (IV) oxide (H-MnO2), the excellent sensitivity and low detection limits (0.243 and 0.692 μg/L respectively) were produced. Based on the proposed method, 25 water and 119 beverage samples were investigated. DBP was found in all water samples and 65.5% of beverage samples, with the concentrations varying in 16.5–32.1 μg/L and 0–553 μg/L, respectively. In addition, the mean concentration (22.9 μg/L) in waters was decreased significantly compared with that detected in 2016 (43.5 μg/L) by our Lab. For beverages, a similar phenomenon was observed by the measured concentrations from coffee. Furthermore, the potential ecological risks of DBP were evaluated, the results indicated that human activities had caused serious pollution and high risks to the local aquatic ecosystem. On the other hand, the results of health risk assessment suggested that DBP in beverages might not cause obvious side effects to local residents.