Cloning and Functional Analysis of Two Ca2+-Binding Proteins (CaBPs) in Response to Cyantraniliprole Exposure in Bemisia tabaci (Hemiptera: Aleyrodidae)
Cloning and Functional Analysis of Two Ca2+-Binding Proteins (CaBPs) in Response to Cyantraniliprole Exposure in Bemisia tabaci (Hemiptera: Aleyrodidae)
复制标题
烟粉虱（半翅目：粉虱科）对氰虫酰胺暴露的两种 Ca2 结合蛋白 (CaBP) 的克隆和功能分析
DOI：
10.1021/acs.jafc.9b04028
复制
发表时间：
2019-10-09
影响因子：
6.1
通讯作者：
Chu, Dong
中科院分区：
文献类型：
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作者：
Guo, Lei;Li, Changyou;Chu, Dong
钙结合蛋白（Ca2+ binding proteins，CaBPs）是一类广泛分布的钙离子传感中继蛋白，参与调节细胞内钙稳态等多种细胞过程。双酰胺杀虫剂如氰虫苯甲酰胺通过破坏肌细胞中的Ca2+稳态来杀死昆虫。然而，很少有人注意到CaBPs在响应杀虫剂中的作用。本研究在烟粉虱Bemisia tabaci（Gennadius）（半翅目：粉虱科）中鉴定了两个CaBP基因（BtCaBP 1和BtCaBP 2），并研究了它们对氰虫苯甲酰胺的响应功能。体外表达BtCaBP 1和BtCaBP 2后，Ca2+成像和细胞毒性实验结果显示，BtCaBP 1和BtCaBP 2的过表达稳定了氰虫苯甲酰胺作用后细胞质中的Ca2+浓度，降低了氰虫苯甲酰胺对Sf 9细胞的毒性。然而，在体内敲低BtCaBP 1或BtCaBP 2显著增加了氰虫苯甲酰胺对B的毒性。烟粉虱综上所述，这些结果提供了证据表明，BtCaBP 1和BtCaBP 2通过稳定粉虱中的Ca2+浓度在响应氰虫苯甲酰胺暴露中发挥作用。
Ca2+-binding proteins (CaBPs) are widely distributed as Ca2+ sensor relay proteins that regulate various cellular processes, including Ca2+ homeostasis. Diamide insecticides such as cyantraniliprole kill insects by disrupting the Ca2+ homeostasis in muscle cells. However, less attention has been paid to the roles of CaBPs in response to insecticides. In this study, two CaBP genes (BtCaBP1 and BtCaBP2) were identified in the whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), and their functions in response to cyantraniliprole were investigated. After expression of BtCaBP1 and BtCaBP2 in vitro, the results of Ca2+ imaging and cytotoxicity assay revealed that the overexpression of each of the BtCaBPs stabilized Ca2+ concentration in the cytoplasm after exposure to cyantraniliprole and decreased the toxicity of cyantraniliprole against Sf9 cells. However, the knockdown of BtCaBP1 or BtCaBP2 in vivo significantly increased the toxicity of cyantraniliprole to B. tabaci. Taken together, these results provide evidence that BtCaBP1 and BtCaBP2 play a role in response to cyantraniliprole exposure through stabilization of Ca2+ concentration in whiteflies.