Apical accumulation of rho in the neural plate is important for neural plate cell shape change and neural tube formation
Apical accumulation of rho in the neural plate is important for neural plate cell shape change and neural tube formation
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DOI：
10.1091/mbc.e07-12-1286
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发表时间：
2008-05-01
影响因子：
3.3
通讯作者：
Yonemura, Shigenobu
中科院分区：
文献类型：
--
作者：
Kinoshita, Nagatoki;Sasai, Noriaki;Yonemura, Shigenobu
虽然Rho-GTP酶是众所周知的细胞骨架重组的调节者，但它们在体内的分布和生理功能仍然难以捉摸。在本研究中，我们发现在发育中的鸡胚胎中，Rho在神经管形成过程中神经板的折叠过程中有明显的顶端积累，与激活的肌球蛋白II的积累相似。Rho和肌球蛋白II的积累和生化激活的时间与神经管形成的动力学一致。抑制Rho干扰其顶端堆积，导致神经管形成缺陷，神经板形态异常。Rho的持续激活也改变了神经管的形成。这些结果表明，Rho的正确时空调控对神经管的形态发生至关重要。此外，我们还发现一个关键的形态发生信号通路Wnt/PCP通路参与了Rho在神经板中的顶端积累和细胞形状的调节，提示该信号可能是Rho在神经管形成中的时空调节因子。
Although Rho-GTPases are well-known regulators of cytoskeletal reorganization, their in vivo distribution and physiological functions have remained elusive. In this study, we found marked apical accumulation of Rho in developing chick embryos undergoing folding of the neural plate during neural tube formation, with similar accumulation of activated myosin II. The timing of accumulation and biochemical activation of both Rho and myosin II was coincident with the dynamics of neural tube formation. Inhibition of Rho disrupted its apical accumulation and led to defects in neural tube formation, with abnormal morphology of the neural plate. Continuous activation of Rho also altered neural tube formation. These results indicate that correct spatiotemporal regulation of Rho is essential for neural tube morphogenesis. Furthermore, we found that a key morphogenetic signaling pathway, the Wnt/PCP pathway, was implicated in the apical accumulation of Rho and regulation of cell shape in the neural plate, suggesting that this signal may be the spatiotemporal regulator of Rho in neural tube formation.