Emergence and development of gut motility in the chicken embryo.
Emergence and development of gut motility in the chicken embryo.
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DOI：
10.1371/journal.pone.0172511
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Asnacios A
中科院分区：
文献类型：
--
作者：
Chevalier NR;Fleury V;Dufour S;Proux-Gillardeaux V;Asnacios A
胃肠道通过消化输送食物团。肠道运动在发育中的胚胎早期就开始了，远远早于有机体所需的营养。我们提出了一个全面的运动学研究的出现和生理发展的肠道运动在所有地区的下消化道的鸡胚胎从胚胎天E5到E9。我们的特点是运动出现时间，传播模式，速度，频率和振幅的波。我们发现，出现一个不间断的圆环平滑肌与传播收缩波的外观，在E6在后肠和中肠，并在E9在盲肠阑尾。我们发现，在这些阶段的消化不良是严重依赖于钙，是不介导的神经元作为肠道运动是不敏感的河豚毒素和发生在后肠神经元的情况下。我们进一步证明了运动性也在离体器官培养中成熟。我们将我们的研究结果与现有的关于斑马鱼、小鼠和人类运动发育的文献进行比较，并讨论了它们与鸡胚胎肠道在这些阶段发生的其他主要发育事件的时间关系。我们的工作为进一步研究这一重要动物模型的运动发育奠定了基础。
The gastrointestinal tract transports the food bolus by peristalsis. Gut motility starts at an early age in the developing embryo, well before it is required for nutrition of the organism. We present a comprehensive kinematic study of the emergence and physiological development of gut motility in all regions of the lower digestive tract of the chicken embryo from embryonic days E5 through E9. We characterized motility emergence time, propagation patterns, speed, frequency and amplitude of peristalsis waves. We found that the emergence of an uninterrupted circular ring of smooth muscle correlated with the appearance of propagative contractile waves, at E6 in the hindgut and midgut, and at E9 in the caecal appendix. We show that peristalsis at these stages is critically dependent on calcium and is not mediated by neurons as gut motility is insensitive to tetrodotoxin and takes place in the hindgut in the absence of neurons. We further demonstrate that motility also matures in ex-vivo organ culture. We compare our results to existing literature on zebrafish, mouse and human motility development, and discuss their chronological relationship with other major developmental events occurring in the chicken embryonic gut at these stages. Our work sets a baseline for further investigations of motility development in this important animal model.