Effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides
Effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides
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DOI：
10.1271/bbb.70689
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发表时间：
2008-03-01
影响因子：
1.6
通讯作者：
Fukuda, Kiyoharu
中科院分区：
文献类型：
--
作者：
Yoshida, Makoto;Liu, Yuan;Fukuda, Kiyoharu
研究了纤维素结晶度、半纤维素和木质素对芒果酶解成单糖的影响。将风干生物质用球磨粉碎，通过一系列筛目尺寸为250~355微米、150~250微米、63~150微米和63微米的一系列筛分，将粉末分成四个级分，每个级分用商用纤维素酶和β-葡萄糖苷酶进行水解。随着底物结晶度的降低，单糖的产率增加。添加木聚糖酶提高了戊糖和葡萄糖的产量。亚氯酸钠法脱木素显著提高了纤维素酶的初始水解率，与未经处理的样品相比，单糖的得率更高。用纤维素酶、β-葡萄糖苷酶和木聚糖酶对脱木素中华绒毛虫进行酶解，半纤维素完全水解单糖，葡聚糖的转化率为90.6%。
The effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides were investigated. A air-dried biomass was ground by ball-milling, and the powder was separated into four fractions by passage through a series of sieves with mesh sizes 250-355 mu m, 150-250 mu m, 63-150 mu m, and < 63 mu m. Each fraction was hydrolyzed with commercially available cellulase and beta-glucosidase. The yield of monosaccharides increased as the crystallinity of the substrate decreased. The addition of xylanase increased the yield of both pentoses and glucose. Delignification by the sodium chlorite method improved the initial rate of hydrolysis by cellulolytic enzymes significantly, resulting in a higher yield of monosaccharides as compared with that for untreated samples. When delignified M. sinensis was hydrolyzed with cellulase, beta-glucosidase, and xylanase, hemicellulose was hydrolyzed completely into monosaccharides, and the conversion rate of glucan to glucose was 90.6%.