Atomic force microscopy reveals age-dependent changes in nanomechanical properties of the extracellular matrix of native human menisci: implications for joint degeneration and osteoarthritis.
Atomic force microscopy reveals age-dependent changes in nanomechanical properties of the extracellular matrix of native human menisci: implications for joint degeneration and osteoarthritis.
复制标题
DOI：
10.1016/j.nano.2014.06.010
复制
发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
D'Lima D
中科院分区：
文献类型：
--
作者：
Kwok J;Grogan S;Meckes B;Arce F;Lal R;D'Lima D
随着年龄的增长，由于持续的机械应力伴随着细胞外基质（ECM）中与年龄相关的变化，椎间盘变得更容易变性。然而，年龄相关性骨关节退行性变和骨关节炎（OA）发展之间的机制关系尚未完全了解。我们已经研究了正常的，老化的，和退化的人类视网膜细胞外基质的纳米力学性能，使用原子力显微镜（AFM）。纳米力学剖面揭示了健康年轻组织的独特的差异性定性纳米力学剖面：三个不同区域（外部、中间和内部）之间的弹性模量分布中的突出单峰。健康老年组织表现出类似的差异弹性的三个区域，但具有单峰和双峰分布，包括较高的弹性模量。相比之下，退化的OA组织显示出最宽的分布，没有显著的峰，表明ECM机械性能的异质性显著增加。AFM分析揭示了不同的区域纳米力学概况，基础老化依赖性组织变性和OA。
With aging, the menisci become more susceptible to degeneration due to sustained mechanical stress accompanied by age-related changes in the extracellular matrix (ECM). However, the mechanistic relationship between age-related meniscal degeneration and osteoarthritis (OA) development is not yet fully understood. We have examined the nanomechanical properties of the ECM of normal, aged, and degenerated human menisci using atomic force microscopy (AFM). Nanomechanical profiles revealed a unique differential qualitative nanomechanical profile of healthy young tissue: prominent unimodal peaks in the elastic moduli distribution among three different regions (outer, middle, and inner). Healthy aged tissue showed similar differential elasticity for the three regions but with both unimodal and bimodal distributions that included higher elastic moduli. In contrast, degenerated OA tissue showed the broadest distribution without prominent peaks indicative of substantially increased heterogeneity in the ECM mechanical properties. AFM analysis reveals distinct regional nanomechanical profiles that underlie aging dependent tissue degeneration and OA.