Enhanced arachidonic acid production from Mortierella alpina combining atmospheric and room temperature plasma (ARTP) and diethyl sulfate treatments
Enhanced arachidonic acid production from Mortierella alpina combining atmospheric and room temperature plasma (ARTP) and diethyl sulfate treatments
复制标题
结合常压和室温等离子体 (ARTP) 和硫酸二乙酯处理，提高高山被孢霉的花生四烯酸产量
DOI：
10.1016/j.biortech.2014.11.051
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发表时间：
2015-02-01
影响因子：
11.4
通讯作者：
Wang, Xingguo
中科院分区：
文献类型：
--
作者：
Li, Xiangyu;Liu, Ruijie;Wang, Xingguo
为了获得高产花生四烯酸（ARA）的突变菌株，采用常压和室温等离子体（ARTP）偶联硫酸二乙酯（DES）诱变产油真菌高山Mortierella alpina。采用目视复合过滤操作，在筛选培养基中添加脂肪酸合成酶（FAS）抑制剂蓝绿蛋白和三苯四唑氯（TTC）。突变株D20的ARA产量为5.09 g/L，比原菌株（3.62 g/L）提高了40.61%。总脂肪酸中ARA的相对含量由38.99%提高到45.64%。优化发酵条件后，菌株D20在摇瓶中ARA产率最高，为6.82 g/L。本研究为利用常规随机突变方法获得高产ara菌株提供了合适的策略。(C) 2014 Elsevier Ltd.版权所有。
To obtain mutant strains with higher arachidonic acid (ARA) yields, the oleaginous fungus Mortierella alpina was mutated using atmospheric and room temperature plasma (ARTP) coupled with diethyl sulfate (DES). A visual compound filter operation was used in which a screening medium was supplemented with cerulenin, an inhibitor of fatty acid synthase (FAS), and triphenyltetrazolium chloride (TTC). The mutant strain D20 with an ARA production of 5.09 g/L, a 40.61% increase over the original strain (3.62 g/L), was isolated. The relative ARA content increased from 38.99% to 45.64% of total fatty acids. After optimizing fermentation conditions, the maximum ARA yield (6.82 g/L) for strain D20 was obtained in shake flasks. This work provides an appropriate strategy for obtaining high ARA-yield strains by conventional random mutation methods with an efficient screening assay. (C) 2014 Elsevier Ltd. All rights reserved.