Isolation of amniotic epithelial stem cells.
Isolation of amniotic epithelial stem cells.
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DOI：
10.1002/9780470151808.sc01e03s12
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发表时间：
2007-11-01
影响因子：
--
通讯作者：
C Strom, Stephen
中科院分区：
文献类型：
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作者：
Miki, Toshio;Marongiu, Fabio;C Strom, Stephen
可以从胎盘组织中分离出的许多细胞类型保留了表型可塑性，这使它们成为再生医学的有趣细胞来源。已经报道了从胎盘的不同部分分离干细胞的几种方法。本单元描述了一个详细而简单的方案，用于从人类足月胎盘中选择性分离羊水上皮细胞，而不干扰间充质层。我们还介绍了一种简单的密度分离技术，用于富集SSEA-4阳性细胞的群体。许多可以从胎盘组织中分离的细胞类型保留了表型可塑性，使其成为再生医学的有趣来源。已经报道了从胎盘的不同部分分离干细胞的几种方法。本单元描述了一个详细而简单的协议，选择性分离羊膜上皮细胞从人类足月胎盘不干扰间充质层。我们还介绍了一种简单的密度分离技术，用于富集SSEA-4阳性细胞的群体。
Many of the cell types that can be isolated from placental tissues retain phenotypic plasticity that makes them an interesting source of cells for regenerative medicine. Several procedures for the isolation of stem cells from different parts of the placenta have been reported. This unit describes a detailed and simple protocol for the selective isolation of amniotic epithelial cells from human term placenta without disturbing the mesenchymal layer. We also introduce a simple density separation technique for the enrichment of the population for SSEA-4 positive cells.Many of the cell types that can be isolated from placental tissues retain phenotypic plasticity that make them an interesting source for regenerative medicine. Several procedures for the isolation of stem cells from different parts of the placenta have been reported. This unit describes a detailed and simple protocol for the selective isolation of amniotic epithelial cells from human term placenta without disturbing the mesenchymal layer. We also introduce a simple density separation technique for the enrichment of the population for SSEA-4 positive cells.