Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death
Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death
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DOI：
10.1016/0092-8674(95)90150-7
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发表时间：
1995-12-29
期刊：
影响因子：
64.5
通讯作者：
Rubin, GM
中科院分区：
文献类型：
--
作者：
Hay, BA;Wassarman, DA;Rubin, GM
细胞凋亡是生物体消除多余或有害细胞的一种机制。细胞死亡调节蛋白REAPER（RPR）在发育中的果蝇眼睛中的表达会导致细胞过度死亡而导致眼睛变小。我们发现，螺纹（th）的突变是RPR诱导的细胞死亡的显性增强子，th编码一种与杆状病毒凋亡抑制剂（IAP）同源的蛋白质，我们称之为果蝇IAP 1（DIAP 1）。DIAP 1或相关蛋白质DIAP 2在眼中的过表达抑制正常发生的细胞死亡以及由于rpr或头部过度表达引起的细胞死亡。IAP死亡预防活性定位于N-末端杆状病毒IAP重复序列，这是在病毒和细胞蛋白中发现的与死亡预防相关的基序。
Apoptotic cell death is a mechanism by which organisms eliminate superfluous or harmful cells. Expression of the cell death regulatory protein REAPER (RPR) in the developing Drosophila eye results in a small eye owing to excess cell death. We show that mutations in thread (th) are dominant enhancers of RPR-induced cell death and that th encodes a protein homologous to baculovirus inhibitors of apoptosis (IAPs), which we call Drosophila IAP1 (DIAP1). Overexpression of DIAP1 or a related protein, DIAP2, in the eye suppresses normally occurring cell death as well as death due to overexpression of rpr or head in volution defective. IAP death-preventing activity localizes to the N-terminal baculovirus IAP repeats, a motif found in both viral and cellular proteins associated with death prevention.