Enrichment of alpha-linolenic acid in rodent diet reduced oxidative stress and inflammation during myocardial infarction
Enrichment of alpha-linolenic acid in rodent diet reduced oxidative stress and inflammation during myocardial infarction
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DOI：
10.1016/j.freeradbiomed.2020.11.025
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发表时间：
2021-01-01
影响因子：
7.4
通讯作者：
Lee, Jetty Chung-Yung
中科院分区：
文献类型：
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作者：
Leung, Kin Sum;Galano, Jean-Marie;Lee, Jetty Chung-Yung
心肌梗塞（MI）是由心脏缺血引起的不可逆事件，可能致命。研究报告称，增加 n-3 多不饱和脂肪酸 (PUFA)（即二十碳五烯酸和二十二碳六烯酸）的摄入量可以降低心血管疾病的风险并降低心肌梗死的发病率。尽管如此，饮食中植物n-3-PUFA（例如α-亚麻酸（ALA））的心脏保护作用尚无定论。在这项研究中，Sprague Dawley 大鼠补充富含 ALA 的亚麻籽 (FS) 和亚麻籽油 (FSO) 的等热量饮食以及正常饲料（对照），持续 4 周。 MI是通过注射异丙肾上腺素（ISO）诱导的。结果表明，所有富含 ALA 的饮食都具有针对心肌梗死的心脏保护作用。与 ISO 相比，心脏与体重的比率、血浆 LDH 活性和血浆 cTnI 均降低，并且在 FS 饮食中尤为显着。与对照组相比，ALA 饮食在组织和血浆中的 ALA 和 EPA 均上调，并且仍高于 ISO 组。值得注意的是，与 ISO 相比，富含 ALA 饮食的组织和血浆中 LOX 介导的 HETE 降低，而 LOX 介导的 HDHA 升高。此外，与对照组相比，补充富含 ALA 饮食的 MI 大鼠的组织和血浆中花生四烯酸的非酶氧化产物（包括 15-F-2t-IsoP）均减少，而来自 n-3 PUFA（包括 F-4-NeuroPs、PhytoPs 和 PhytoFs）的非酶氧化产物则升高。富含 ALA 的饮食，特别是亚麻籽，可以降低炎症细胞因子（即 IL-1β、IL-6 和 TNFα）的基因表达，并防止心脏组织中抗氧化剂过氧化氢酶的下调。总之，富含 ALA 的饮食可能通过调节 n-3 PUFA 自动氧化的抗炎和抗氧化介质发挥心脏保护作用。
Myocardial infarction (MI) is an irreversible event caused by cardiac ischemia and may be fatal. Studies reported that increased intake of n-3 polyunsaturated fatty acids (PUFA) namely, eicosapentaenoic acid and docosahexaenoic acid reduce the risk of cardiovascular disease and lower the incidence of MI. Nonetheless, the cardioprotective effect of plant n-3-PUFA such as alpha-linolenic acid (ALA) in the diet is not conclusive. In this study, Sprague Dawley rats were supplemented with isocaloric diets enriched with ALA rich flaxseed (FS) and flaxseed oil (FSO), and normal chow (Control) for 4 weeks. MI was induced by isoproterenol (ISO) injection. Results showed that all ALA-enriched diets displayed cardioprotection against MI. The heart to body weight ratio, plasma LDH activity and plasma cTnI were reduced compared to ISO and was prominent in FS diet. ALA and EPA were up-regulated in both tissues and plasma by ALA-diets compared to Control and remained higher than ISO groups. Notably, LOX-mediated HETEs decreased whereas LOX-mediated HDHAs were elevated in both tissues and plasma of ALA-enriched diets compared to ISO. In addition, non-enzymatic oxidized products from arachidonic acid including 15-F-2t-IsoP were reduced in both tissues and plasma of MI rats supplemented with ALA-enriched diets while those from n-3 PUFAs including F-4-NeuroPs, PhytoPs and PhytoFs were elevated compared to control. ALA-enriched diets particularly flaxseed reduced gene expressions of inflammatory cytokines namely IL-1 beta, IL-6 and TNF alpha and prevented the down regulation of antioxidant catalase in the heart tissues. In conclusion ALA-enriched diets potentially exerted cardioprotection through the regulation of anti-inflammatory and antioxidative mediators from n-3 PUFA autooxidation.