Ly6G deficiency alters the dynamics of neutrophil recruitment and pathogen capture during Leishmania major skin infection.
Ly6G deficiency alters the dynamics of neutrophil recruitment and pathogen capture during Leishmania major skin infection.
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DOI：
10.1038/s41598-021-94425-9
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发表时间：
2021-07-23
影响因子：
4.6
通讯作者：
Müller AJ
中科院分区：
文献类型：
--
作者：
Kleinholz CL;Riek-Burchardt M;Seiß EA;Amore J;Gintschel P;Philipsen L;Bousso P;Relja B;Schraven B;Handschuh J;Mohr J;Müller AJ
中性粒细胞是最早被募集到感染部位的免疫细胞类型之一，在感染部位它们可以通过吞噬作用和细胞毒性机制控制病原体。细胞内病原体，如利什曼原虫可以劫持中性粒细胞，建立一个有效的感染。然而，在感染部位中性粒细胞与病原体和其他细胞的动态相互作用还不完全清楚。在这里，我们研究了Ly6G的作用，Ly6G是人类CD177蛋白的同源物，已被证明与细胞粘附分子相互作用，并作为小鼠中性粒细胞的真正标志物。我们发现，Ly6G缺乏降低了中性粒细胞招募到感染部位的初始感染率。虽然L.主要由随后募集的单核细胞与中性粒细胞物质的伴随摄取紧密相关，该过程不被中性粒细胞的Ly6G缺乏改变。相反，我们通过活体双光子显微镜观察到Ly6 G缺陷的中性粒细胞进入感染部位，初始募集动力学延迟。因此，我们得出结论，通过促进中性粒细胞有效进入感染部位的能力，Ly6G有助于免疫系统早期参与细胞内病原体。
Neutrophils represent one of the first immune cell types recruited to sites of infection, where they can control pathogens by phagocytosis and cytotoxic mechanisms. Intracellular pathogens such as Leishmania major can hijack neutrophils to establish an efficient infection. However the dynamic interactions of neutrophils with the pathogen and other cells at the site of the infection are incompletely understood. Here, we have investigated the role of Ly6G, a homolog of the human CD177 protein, which has been shown to interact with cell adhesion molecules, and serves as a bona fide marker for neutrophils in mice. We show that Ly6G deficiency decreases the initial infection rate of neutrophils recruited to the site of infection. Although the uptake of L. major by subsequently recruited monocytes was tightly linked with the concomitant uptake of neutrophil material, this process was not altered by Ly6G deficiency of the neutrophils. Instead, we observed by intravital 2-photon microscopy that Ly6G-deficient neutrophils entered the site of infection with delayed initial recruitment kinetics. Thus, we conclude that by promoting neutrophils’ ability to efficiently enter the site of infection, Ly6G contributes to the early engagement of intracellular pathogens by the immune system.
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影响因子：
6.7
作者：
Hurrell BP;Schuster S;Grün E;Coutaz M;Williams RA;Held W;Malissen B;Malissen M;Yousefi S;Simon HU;Müller AJ;Tacchini-Cottier F
通讯作者：
Tacchini-Cottier F
影响因子：
6.7
作者：
Bruns S;Kniemeyer O;Hasenberg M;Aimanianda V;Nietzsche S;Thywissen A;Jeron A;Latgé JP;Brakhage AA;Gunzer M
通讯作者：
Gunzer M
影响因子：
20.3
作者：
Bai, Ming;Grieshaber-Bouyer, Ricardo;Nigrovic, Peter A.
通讯作者：
Nigrovic, Peter A.
DOI：
10.4049/jimmunol.1200385
发表时间：
2012-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Griffin GK;Newton G;Tarrio ML;Bu DX;Maganto-Garcia E;Azcutia V;Alcaide P;Grabie N;Luscinskas FW;Croce KJ;Lichtman AH
通讯作者：
Lichtman AH
影响因子：
5.4
作者：
Charmoy, Melanie;Hurrell, Benjamin P.;Sacks, David L.
通讯作者：
Sacks, David L.