FGF-8 EXPRESSION IN THE POST-GASTRULATION MOUSE SUGGESTS ROLES IN THE DEVELOPMENT OF THE FACE, LIMBS AND CENTRAL-NERVOUS-SYSTEM
FGF-8 EXPRESSION IN THE POST-GASTRULATION MOUSE SUGGESTS ROLES IN THE DEVELOPMENT OF THE FACE, LIMBS AND CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1016/0925-4773(94)90022-1
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发表时间：
1994-11-01
影响因子：
2.6
通讯作者：
MACARTHUR, CA
中科院分区：
文献类型：
--
作者：
HEIKINHEIMO, M;LAWSHE, A;MACARTHUR, CA
成纤维细胞生长因子8（Fgf - 8）是成纤维细胞生长因子（FGF）家族的成员，最初在一种乳腺癌细胞系中被鉴定为一种雄激素诱导的生长因子。原始Fgf - 8转录本的可变剪接产生三种信使RNA，它们编码分泌型FGF - 8蛋白异构体，这些异构体仅在其成熟的氨基末端有所不同。通过Northern杂交分析，在小鼠妊娠第10天到第12天存在Fgf - 8 RNA，这表明Fgf - 8在原肠胚形成后的发育过程中正常发挥作用。为了描述小鼠发育过程中Fgf - 8表达的时间、空间和异构体特异性方面的特征，我们在妊娠第8天到第16天之间进行了原位杂交和核糖核酸酶保护实验。在妊娠第9天，在第一鳃弓的表面外胚层、额鼻突、前脑以及中脑 - 后脑连接处首次检测到Fgf - 8的表达。在妊娠第10 - 12天，在前后肢芽的表面外胚层、鼻窝和鼻咽、漏斗以及端脑、间脑和后脑检测到Fgf - 8的表达。Fgf - 8在发育中的后肢的表达持续到妊娠第13天，之后则检测不到。核糖核酸酶保护实验显示，在妊娠第10 - 12天存在编码所有三种FGF - 8异构体的RNA。这些结果揭示了Fgf - 8在发育中的小鼠中独特的时空表达模式，并表明这种FGF在原肠胚形成后的小鼠胚胎的外胚层分化的多个区域中发挥作用。
Fgf-8 is a member of the fibroblast growth factor (FGF) family that was initially identified as an androgen-inducible growth factor in a mammary carcinoma cell line. Alternative splicing of the primary Fgf-8 transcript results in three messenger RNAs which code for secreted FGF-8 protein isoforms that differ only in their mature amino termini. Fgf-8 RNA is present from day 10 through 12 of murine gestation when analyzed by northern blot analysis, suggesting that Fgf-8 normally functions during post-gastrulation development. To characterize the temporal, Spatial and isoform-specific aspects of Fgf-8 expression during mouse development, we performed in situ hybridization and ribonuclease protection assays between the days 8 and 16 of gestation. Fgf-8 expression is first detected at day 9 of gestation in the surface ectoderm of the first branchial arches, the frontonasal process, the forebrain and the midbrain-hindbrain junction. At days 10-12 of gestation, Fgf-8 expression is detected in the surface ectoderm of the forelimb and hindlimb buds, in the nasal pits and nasopharynx, in the infundibulum and in the telencephalon, diencephalon and metencephalon. Fgf-8 expression continues in the developing hindlimbs through day 13 of gestation but is undetectable thereafter. Ribonuclease protection assays reveal that RNAs coding for all three FGF-8 isoforms are present at days 10-12 of gestation. These results reveal a unique temporal and spatial pattern of Fgf-8 expression in:the developing mouse and suggest a role for this FGF in multiple regions of ectodermal differentiation in the post-gastrulation mouse embryo.