Asymmetric localization of the CDC25B phosphatase to the mother centrosome during interphase
Asymmetric localization of the CDC25B phosphatase to the mother centrosome during interphase
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DOI：
10.4161/cc.7.3.5295
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发表时间：
2008-02-01
期刊：
影响因子：
4.3
通讯作者：
Ducommun, Bernard
中科院分区：
文献类型：
--
作者：
Boutros, Rose;Ducommun, Bernard
细胞周期蛋白依赖性激酶（CDK）激活磷酸酶CDC 25 B定位于中心体，其活性受到包括Aurora A和CHK 1在内的几种激酶的正调控和负调控。我们最近的数据还表明，CDC 25 B在中心体复制周期和微管成核的间期，似乎涉及招聘的γ-微管蛋白的中心体的作用。在本研究中，我们报道了CDC 25 B，沿着CHK 1，CDK 1和WEE 1，从S期到G（2）期不对称地分布在母体中心体周围，到G（2）期晚期，随着中心体的成熟，逐渐均匀地分布在两个中心体上。我们进一步证明，siRNA抑制CDC 25 B导致细胞聚集在G（2）期，具有两个分离的中心体，每个中心体仅包含一个中心粒，这表明中心粒复制需要CDC 25 B。我们建议，本地化的关键细胞周期调节母中心体，确保中心体复制和细胞分裂周期之间的同步。
The cyclin-dependent kinase (CDK)-activating phosphatase CDC25B, localises to the centrosomes where its activity is both positively and negatively regulated by several kinases including Aurora A and CHK1. Our recent data also demonstrate a role for CDC25B in the centrosome duplication cycle and microtubule nucleation in interphase that appears to involve the recruitment of gamma-tubulin to the centrosomes. In the present study, we report that CDC25B, along with CHK1, CDK1 and WEE1, localise asymmetrically around the mother centrosome from S to G(2)-phases, and gradually become evenly distributed to the two centrosomes by late G(2) phase, concomitant with centrosome maturation. We further demonstrate that siRNA inhibition of CDC25B results in an accumulation of cells in G(2) phase with two separated centrosomes, each containing only a single centriole, suggesting a requirement for CDC25B in centriole duplication. We propose that the localisation of key cell cycle regulators to the mother centrosome ensures synchrony between the centrosome duplication and cell division cycles.